Adenosine A1 receptor agonist treatment up-regulates rat brain metabotropic glutamate receptors

被引:17
作者
Albasanz, JL [1 ]
León, D [1 ]
Ruíz, MA [1 ]
Fernández, M [1 ]
Martín, M [1 ]
机构
[1] Univ Castilla La Mancha, Ctr Reg Invest Biomed, Fac Ciencias Quim, Dept Quim Inorgan Organ & Bioquim,Area Bioquim, E-13071 Ciudad Real, Spain
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2002年 / 1593卷 / 01期
关键词
adenosine A(1) receptor; metabotropic glutamate receptor; phospholipase C; up-regulation; rat brain;
D O I
10.1016/S0167-4889(02)00330-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chronic R-N-6-phenylisopropiladenosine (R-PIA) subcutaneous injection for 6 days significantly increased total glutamate receptor number (180% of control) in rat brain synaptic plasma membranes (SPM), without affecting receptor affinity. A higher increase in metabotropic glutamate (mGlu) receptor number (258% of control) was also detected, indicating that mGlu is the main type of glutamate receptor affected by this treatment. On the other hand, the observed increase in basal and calcium- and Gpp(NH)p-stimulated phospholipase C (PLC) activity after treatment was associated with a significant increase in PLC beta(1) isoform, detected in SPM by immunoblotting assays. Moreover, an increase in PLC activity stimulation with trans-ACPD, in the absence and in the presence of Gpp(NH)p, was detected after R-PIA treatment. These results show that mGlu. receptors and its effector system, PLC activity, are up-regulated by chronic exposure to an adenosine A, receptor agonist and suggest the existence of a cross-talk mechanism between both signal transduction pathways in rat brain. (C) 2002 Elsevier Science B.V All rights reserved.
引用
收藏
页码:69 / 75
页数:7
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