Sensitivity-enhanced E.COSY-type HSQC experiments for accurate measurements of one-bond 15N-1HN and 15N-13C′ and two-bond 13C′-1HN residual dipolar couplings in proteins

被引：16

作者：

Ding, KY ^{[1
]}

Gronenborn, AM ^{[1
]}

机构：

[1] NIDDKD, Chem Phys Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF MAGNETIC RESONANCE | 2002年 / 158卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

residual dipolar couplings; E.COSY; proteins; structural genomics;

D O I：

10.1016/S1090-7807(02)00024-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel E.COSY-type HSQC experiments are presented for the accurate measurement of one-bond N-15-H-1(N) and N-15-C-13' and two-bond C-13'-H-1(N) residual dipolar couplings in proteins. Compared with existing experiments, the (delta,J)-E.COSY experiments described here are composed of fewer pulses and the resulting spectra exhibit 1.4 times the sensitivity of coupled HSQC spectra. Since residual dipolar couplings play increasingly important roles in structural NMR, the proposed methods should find wide spread application for structure determination of proteins and other biological macromolecules. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：173 / 177

页数：5

共 29 条

[1] Dipolar couplings in macromolecular structure determination [J].