Analysis of O-glucuronide conjugates in urine by electrospray ion trap mass spectrometry

被引：43

作者：

Gu, JK ^{[1
]}

Zhong, DF ^{[1
]}

Chen, XY ^{[1
]}

机构：

[1] Shenyany Pharmaceut Univ, Lab Drug Metab & Pharmacokinet, Shenyang 110015, Peoples R China

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1999年 / 365卷 / 06期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1007/s002160051521

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The application of electrospray ionization (ESI) ion trap mass spectrometry in the characterization of O-glucuronide conjugates of some drugs in urine is described. The conjugated metabolites formed in rabbit and human were separated by reversed-phase high-performance liquid chromatography (HPLC) and characterized by multi-stage mass spectrometry (MSn) experiments in negative ion mode. The ESI mass spectra showed a deprotonated molecule [M-H](-), which was chosen as precursor ion. Collision-induced dissociation (CID) of [M-H](-) in MSn experiments resulted in the appearance of glucuronate 'fingerprint' ions at m/z 175 and 113 as well as prominent aglycone ions which were the same as those produced from authentic specimens. This information can be used to identify this type of compound directly without the need for derivatization or hydrolysis of enzymes, providing a rapid and specific method for guiding the isolation and characterization of similar compounds in complex matrices with LC/MS.

引用

页码：553 / 558

页数：6

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