Cytokine profile of iron-laden macrophages: Implications for cellular magnetic resonance imaging

被引：77

作者：

Siglienti, I

Bendszus, M

Kleinschnitz, C

Stoll, G

机构：

[1] Univ Wurzburg, Dept Neurol, D-97080 Wurzburg, Germany

[2] Univ Wurzburg, Dept Neuroradiol, D-97080 Wurzburg, Germany

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2006年 / 173卷 / 1-2期

关键词：

SPIO; USPIO; cytokines; macrophages; magnetic resonance imaging;

D O I：

10.1016/j.jneuroim.2005.11.011

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Superparamagnetic iron oxide (SPIO/USPIO) particles are a promising new tool to label cells for in vivo monitoring of their migration into the nervous system by magnetic resonance imaging (MRI). Upon systemic application, SPIO/USPIO particles are preferentially internalized by macrophages. It is unclear whether this affects their immunological profile. We tested the cytokine production of rat and mouse macrophages in vitro and found that internalization of SPIO/USPIO shifted macrophages towards an anti-inflammatory, less responsive phenotype by enhancing interleukin (IL)-10 and inhibiting tumor necrosis factor (TNF)-alpha production. During macrophage interaction with T-cells IL-12p40 secretion was inhibited. Based on our in vitro findings, potential immunomodulatory effects of SPIO/USPIO particles in vivo warrant further investigation. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：166 / 173

页数：8

共 40 条

[1] Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging [J].