Design of a filamentous polymeric scaffold for in vivo guided angiogenesis

被引:20
作者
Gafni, Yossi
Zilberman, Yoram
Ophir, Zohar
Abramovitch, Rinat
Jaffe, Michael
Gazit, Zulma
Domb, Avraham, Jr.
Gazit, Dan [1 ]
机构
[1] Hebrew Univ Jerusalem, Hadassah Med Ctr, Skeletal Biotechnol Lab, Jerusalem, Israel
[2] New Jersey Inst Technol, Newark, NJ 07102 USA
[3] Hadassah Univ Hosp, HBRC, MRI MRS Lab, IL-91120 Jerusalem, Israel
[4] Hadassah Univ Hosp, Goldyne Savad Inst Gene Therapy, IL-91120 Jerusalem, Israel
[5] Hebrew Univ Jerusalem, Sch Pharm, Dept Med Chem & Nat Prod, Jerusalem, Israel
来源
TISSUE ENGINEERING | 2006年 / 12卷 / 11期
关键词
D O I
10.1089/ten.2006.12.3021
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Angiogenesis is mandatory for reperfusion of viable tissues, and lack of vascularization may cause ischemia. The increasing disparity between the demand and availability of adequate substitutes for small-diameter human blood vessels has prompted an intensive search for artificial materials or biological allograft tissues, both of which usually fail in the long term. The objective of this study was to pioneer a novel model for in vivo guided angiogenesis based on a specific design process of a filamentous polymeric scaffold with endothelial cells in a 3-dimensional culture system. To our knowledge, this is the first report of an in vivo guided angiogenesis approach based on a 2-step model, composed of endothelial cells and a filamentous polymeric scaffold framework. Endothelial cells that had been cultured on a specifically designed filamentous polymeric scaffold within a regulated dynamic tissue culture system were shown in vivo to induce guided angiogenesis. Cells seeded on a biodegradable polymeric scaffold were implanted into mice. On day 28 after implantation, analysis revealed a guided angiogenic process along the path of the implanted polymeric scaffold as well as initial evidence for early maturation of engineered vessels, allowing red blood cells to flow through the forming lumina of new vessels as the polymer degraded. The authors conclude that in vivo guided angiogenesis can be achieved by combining endothelial cells with biodegradable filamentous polymeric scaffolds and that this model can lay the cornerstone for vascular engineering and future development of clinically available protocols aimed to treat life-threatening cardiovascular conditions.
引用
收藏
页码:3021 / 3034
页数:14
相关论文
共 39 条
  • [1] Analysis of subcutaneous angiogenesis by gradient echo magnetic resonance imaging
    Abramovitch, R
    Frenkiel, D
    Neeman, M
    [J]. MAGNETIC RESONANCE IN MEDICINE, 1998, 39 (05) : 813 - 824
  • [2] Abramovitch R, 1999, CANCER RES, V59, P5012
  • [3] Endothelial/pericyte interactions
    Armulik, A
    Abramsson, A
    Betsholtz, C
    [J]. CIRCULATION RESEARCH, 2005, 97 (06) : 512 - 523
  • [4] Molecular imaging of the skeleton: Quantitative real-time bioluminescence monitoring gene expression in bone repair and development
    Bar, I
    Zilberman, Y
    Zeira, E
    Galun, E
    Honigman, A
    Turgeman, G
    Clemens, T
    Gazit, Z
    Gazit, D
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 2003, 18 (03) : 570 - 578
  • [5] Visualizing gene expression in living mammals using a bioluminescent reporter
    Contag, CH
    Spilman, SD
    Contag, PR
    Oshiro, M
    Eames, B
    Dennery, P
    Stevenson, DK
    Benaron, DA
    [J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1997, 66 (04) : 523 - 531
  • [6] VEGF regulates cell behavior during vasculogenesis
    Drake, CJ
    LaRue, A
    Ferrara, N
    Little, CD
    [J]. DEVELOPMENTAL BIOLOGY, 2000, 224 (02) : 178 - 188
  • [7] Endothelialized microvasculature based on a biodegradable elastomer
    Fidkowski, C
    Kaazempur-Mofrad, MR
    Borenstein, J
    Vacanti, JP
    Langer, R
    Wang, YD
    [J]. TISSUE ENGINEERING, 2005, 11 (1-2): : 302 - 309
  • [8] Freed L.E., 2000, PRINCIPLES TISSUE EN, P143
  • [9] Gene therapy platform for bone regeneration using an exogenously regulated, AAV-2-based gene expression system
    Gafni, Y
    Pelled, G
    Zilberman, Y
    Turgeman, G
    Apparailly, F
    Yotvat, H
    Galun, E
    Gazit, Z
    Jorgensen, C
    Gazit, D
    [J]. MOLECULAR THERAPY, 2004, 9 (04) : 587 - 595
  • [10] Gerhardt H, 2000, DEV DYNAM, V218, P472, DOI 10.1002/1097-0177(200007)218:3<472::AID-DVDY1008>3.0.CO