Immunohistochemical patterns for α- and β-catenin, E- and N-cadherin expression in ovarian epithelial tumors

被引:35
作者
Marques, FR
Fonsechi-Carvasan, GA
Andrade, LALD
Böttcher-Luiz, F
机构
[1] Univ Estadual Campinas, Fac Med Sci, Dept Gynecol & Obstet, Campinas, SP, Brazil
[2] Univ Estadual Campinas, Fac Med Sci, Dept Pathol, Campinas, SP, Brazil
关键词
ovarian neoplasms; cellular adhesion molecules; catenins; cadherins; immunohistochemistry;
D O I
10.1016/j.ygyno.2004.03.037
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Objectives. This study aims were to analyze and compare the E- and N-cadherin, beta- and alpha-catenin expression in benign and malignant epithelial neoplasms of the ovary, correlating with tumor staging, histological grade, and presence of metastases during evolution. Methods. Immunohistochemical reactions were performed on paraffin-embedded tissues and evaluated according to the number of positive, stained cellular structures and reaction intensity for each molecule. Information about histological type and grade, tumoral stage, and disease evolution was obtained from the patients' clinical records. Results. Most of the carcinomas showed more intense beta-catenin reaction (P = 0.02). More than 50% of the endometrioid carcinomas showed increased beta-catenin expression, with a large number of positive cells and more intense staining, being the same also observed for most of the serous benign tumors (P < 0.01). E-cadherin membrane expression was frequently observed in carcinomas without metastasis, whereas cases with metastases in evolution were negative or showing E-cadherin expression only in the cytoplasm (P = 0.04). N-cadherin expression differed according to histological type and grade and alpha-catenin was also related to histological type, but these findings were not conclusive. Conclusions. Increased beta-catenin expression was more frequent in ovarian carcinomas, especially, but not only, in the endometrioid ones. The maintenance of E-cadherin expression in cellular membrane may be an independent marker of good prognosis in ovarian cancer. New studies about N-cadherin and alpha-catenin and their importance during ovarian carcinogenesis will be required. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:16 / 24
页数:9
相关论文
共 23 条
[1]   Clinical significance of α-catenin, collagen IV, and Ki-67 expression in epithelial ovarian cancer [J].
Anttila, M ;
Kosma, VM ;
Ji, HX ;
Wei-Ling, X ;
Puolakka, J ;
Juhola, M ;
Saarikoski, S ;
Syrjanen, K .
JOURNAL OF CLINICAL ONCOLOGY, 1998, 16 (08) :2591-2600
[2]   E-cadherin induces mesenchymal-to-epithelial transition in human ovarian surface epithelium [J].
Auersperg, N ;
Pan, J ;
Grove, BD ;
Peterson, T ;
Fisher, J ;
Maines-Bandiera, S ;
Somasiri, A ;
Roskelley, CD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (11) :6249-6254
[3]   The E-cadherin cell-cell adhesion complex and lung cancer invasion, metastasis, and prognosis [J].
Bremnes, RM ;
Veve, R ;
Hirsch, FR ;
Franklin, WA .
LUNG CANCER, 2002, 36 (02) :115-124
[4]  
BULKHOLM IK, 2000, J PATHOL, V190, P15
[5]   Expression of wild-type alpha-catenin protein in cells with a mutant alpha-catenin gene restores both growth regulation and tumor suppressor activities [J].
Bullions, LC ;
Notterman, DA ;
Chung, LS ;
Levine, AJ .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (08) :4501-4508
[6]   Cell adhesion in tumor invasion and metastasis: loss of the glue is not enough [J].
Cavallaro, U ;
Christofori, G .
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 2001, 1552 (01) :39-45
[7]  
Davidson B, 2000, J PATHOL, V192, P460
[8]  
Davies BR, 1998, HISTOPATHOLOGY, V32, P69
[9]   Prognostication of invasive ductal breast cancer by quantification of E-cadherin immunostaining: the methodology and clinical relevance [J].
Elzagheid, A ;
Kuopio, T ;
Ilmen, M ;
Collan, Y .
HISTOPATHOLOGY, 2002, 41 (02) :127-133
[10]  
Fisher L. D., 1993, BIOESTATISTICS METHO