alpha(2)-adrenoceptor regulation of adenylyl cyclase in CHO cells: dependence on receptor density, receptor subtype and current activity of adenylyl cyclase

被引:78
作者
Pohjanoksa, K
Jansson, CC
Luomala, K
Marjamaki, A
Savola, JM
Scheinin, M
机构
[1] UNIV TURKU, DEPT PHARMACOL & CLIN PHARMACOL, FIN-20520 TURKU, FINLAND
[2] UNIV TURKU, CTR BIOTECHNOL, FIN-20520 TURKU, FINLAND
[3] ORION CORP FARMOS, ORION PHARMA, FIN-20101 TURKU, FINLAND
关键词
alpha(2)-adrenoceptor subtype; CHO cell; H-3]RX821002 binding; adenylyl cyclase; cAMP;
D O I
10.1016/S0014-2999(97)01154-0
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Chinese hamster ovary (CHO) cells stably transfected to express different densities of the human alpha(2A)-, alpha(2B)- and alpha(2C)-adrenoceptor subtypes, were used to characterize the regulation of adenylyl cyclase activity by alpha(2)-adrenoceptor agonists. In isolated cell membranes, activation of alpha(2A)- and alpha(2C)-adrenoceptors did not affect basal enzyme activity, but activation of alpha(2B)-adrenoceptors stimulated adenylyl cyclase activity, The extent of stimulation was dependent on the receptor density and was insensitive to pertussis toxin treatment. In the presence of 10 mu M forskolin all three receptor subtypes mediated inhibition of adenylyl cyclase activity in a pertussis toxin-sensitive manner. In experiments performed with intact cells the same pattern could be seen: the basal production of cAMP was not affected when alpha(2C)-adrenoceptors were activated, but activated alpha(2B)-adrenoceptors mediated stimulation of cAMP production. In the presence of forskolin, both receptor subtypes mediated inhibition of cAMP production. Our results suggest that alpha(2B)-adrenoceptors are coupled to both G(i) and G(s) proteins. The signal transduction pathway to which the receptor is coupled is not dependent on receptor density, but its effect on adenylyl cyclase regulation is dependent on the current activity of adenylyl cyclase. The results also suggest that the alpha(2A)- and alpha(2C)-subtypes are preferentially coupled to G(i) and transduce only inhibition of adenylyl cyclase activity in transfected CHO cells. At low densities of alpha(2C)-adrenoceptors, clonidine was a partial agonist, but in clones expressing high levels of alpha(2C)-adrenoceptors, clonidine acted as a full agonist by inhibiting cAMP accumulation with the same efficacy as (-)-noradrenaline. This demonstrates that receptor reserve can mask partial agonist activity. (C) 1997 Elsevier Science B.V.
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页码:53 / 63
页数:11
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