Two Novel Exopolysaccharides from Bacillus amyloliquefaciens C-1: Antioxidation and Effect on Oxidative Stress

被引：51

作者：

Yang, Haixia ^{[1
]}

Deng, Jianjun ^{[2
]}

Yuan, Yue ^{[1
]}

Fan, Daidi ^{[2
]}

Zhang, Yan ^{[3
]}

Zhang, Ruijuan ^{[1
]}

Han, Bei ^{[1
]}

机构：

[1] Xi An Jiao Tong Univ, Hlth Sci Ctr, Xian 710061, Shaanxi, Peoples R China

[2] NW Univ Xian, Coll Chem Engn, Dept Food Sci & Engn, Shaanxi Key Lab Degradable Biomed Mat, Xian 710069, Peoples R China

[3] Univ Louisville, Sch Med, Dept Neurosci Training, Louisville, KY 40202 USA

来源：

CURRENT MICROBIOLOGY | 2015年 / 70卷 / 02期

基金：

中国博士后科学基金;

关键词：

IN-VITRO; FREE-RADICALS; POLYSACCHARIDES; STRAIN; PURIFICATION; LEVAN;

D O I：

10.1007/s00284-014-0717-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two odorless, water-soluble exopolysaccharide (EPS) fractions, EPS-1 and EPS-2, were isolated from a newly isolated Bacillus amyloliquefaciens strain C-1 and purified by ion exchange and gel chromatography. The purified EPS-1 contained glucose/mannose/galactose/arabinose in a relative proportion of 15:4:2:1, and possessed a molecular weight of 79.6 kDa, while EPS-2 contained only glucose and mannose in a 3:1 ratio, with the molecular weights of 19.8 kDa. The antioxidant activity results showed that EPS-1 exhibited strong reducing power, superoxide radicals (O2-center dot), and hydroxyl free radicals (OH center dot) scavenging activities. For the H2O2-induced injury in HepG2 cells, EPS-1 significantly decreased the formation of reactive oxygen species, intracellular malondialdehyde levels, and restored intracellular superoxide dismutase activity. For EPS-2, there had no detectable antioxidant activities. And all these results collectively showed that as a natural antioxidant, only EPS-1 produced by C-1 had considerable potential to be used as medical compounds or functional additives.

引用

页码：298 / 306

页数：9

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