Identification of RAPD markers linked to a locus involved in quantitative resistance to TYLCV in tomato by Bulked Segregant Analysis

被引：65

作者：

Chague, V

Mercier, JC

Guenard, M

deCourcel, A

Vedel, F

机构：

[1] CLAUSE SEMENCES,STN RECH ST REMY,F-13210 ST REMY PROVENCE,FRANCE

[2] CLAUSE SEMENCES,F-91220 BRETIGNY SUR ORGE,FRANCE

来源：

THEORETICAL AND APPLIED GENETICS | 1997年 / 95卷 / 04期

关键词：

Bulked Segregant Analysis; marker-assisted selection; QTL; RAPD; Tomato Yellow Leaf Curl Virus;

D O I：

10.1007/s001220050611

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In tomato, Bulked Segregant Analysis was used to identify random amplified polymorphic DNA (RAPD) markers linked to a quantitative trait locus (QTL) involved in the resistance to the Tomato Yellow Leaf Curl Virus. F-4 lines were distributed into two pools, each consisting of the most resistant and of the most susceptible individuals, respectively. Both pools were screened using 600 random primers. Four RAPD markers were found to be linked to a QTL responsible for up to 27.7% of the resistance. These markers, localized in the same linkage group within a distance of 17.3 cM, were mapped to chromosome 6 on the tomato RFLP map.

引用

页码：671 / 677

页数：7

共 39 条

[1] LOCALIZATION OF QUANTITATIVE TRAIT LOCI (QTL) FOR AGRONOMIC IMPORTANT CHARACTERS BY THE USE OF A RFLP MAP IN BARLEY (HORDEUM-VULGARE L) [J].