Two novel proteins in the mitochondrial outer membrane mediate β-barrel protein assembly

被引:126
作者
Ishikawa, D
Yamamoto, H
Tamura, Y
Moritoh, K
Endo, T [1 ]
机构
[1] Nagoya Univ, Grad Sch Sci, Dept Chem, Chikusa Ku, Nagoya, Aichi 4648602, Japan
[2] Nagoya Univ, Inst Adv Res, Chikusa Ku, Nagoya, Aichi 4648602, Japan
[3] Nagoya Univ, Core Res Evolut Sci & Technol, Japan Sci & Technol Corp, Chikusa Ku, Nagoya, Aichi 4648602, Japan
关键词
mitochondria; protein import; membrane protein assembly; yeast; translocator;
D O I
10.1083/jcb.200405138
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Mitochondrial outer and inner membranes contain translocators that achieve protein translocation across and/or insertion into the membranes. Recent evidence has shown that mitochondrial beta-barrel protein assembly in the outer membrane requires specific translocator proteins in addition to the components of the general translocator complex in the outer membrane, the TOM40 complex. Here we report two novel mitochondrial outer membrane proteins in yeast, Tom-beta and Tom38/Sam35, that mediate assembly of mitochondrial beta-barrel proteins, Tom40, and/or porin in the outer membrane. Depletion of Tom13 or Tom38/Sam35 affects assembly pathways of the beta-barrel proteins differently, suggesting that they mediate different steps of the complex assembly processes of beta-barrel proteins in the outer membrane.
引用
收藏
页码:621 / 627
页数:7
相关论文
共 19 条
  • [1] The Tim core complex defines the number of mitochondrial translocation contact sites and can hold arrested preproteins in the absence of matrix Hsp70-Tim44
    Dekker, PJT
    Martin, F
    Maarse, AC
    Bomer, U
    Muller, H
    Guiard, B
    Meijer, M
    Rassow, J
    Pfanner, N
    [J]. EMBO JOURNAL, 1997, 16 (17) : 5408 - 5419
  • [2] Functional cooperation and separation of translocators in protein import into mitochondria, the double-membrane bounded organelles
    Endo, T
    Yamamoto, H
    Esaki, M
    [J]. JOURNAL OF CELL SCIENCE, 2003, 116 (16) : 3259 - 3267
  • [3] The Omp85 family of proteins is essential for outer membrane biogenesis in mitochondria and bacteria
    Gentle, I
    Gabriel, K
    Beech, P
    Waller, R
    Lithgow, T
    [J]. JOURNAL OF CELL BIOLOGY, 2004, 164 (01) : 19 - 24
  • [4] Protein transport into mitochondria
    Herrmann, JM
    Neupert, W
    [J]. CURRENT OPINION IN MICROBIOLOGY, 2000, 3 (02) : 210 - 214
  • [5] Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins
    Hill, K
    Model, K
    Ryan, MT
    Dietmeier, K
    Martin, F
    Wagner, R
    Pfanner, N
    [J]. NATURE, 1998, 395 (6701) : 516 - 521
  • [6] The Tim8-Tim13 complex of Neurospora crassa functions in the assembly of proteins into both mitochondrial membranes
    Hoppins, SC
    Nargang, FE
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (13) : 12396 - 12405
  • [7] An essential role of Sam50 in the protein sorting and assembly machinery of the mitochondrial outer membrane
    Kozjak, V
    Wiedemann, N
    Milenkovic, D
    Lohaus, C
    Meyer, HE
    Guiard, B
    Meisinger, C
    Pfanner, N
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (49) : 48520 - 48523
  • [8] Biogenesis of porin of the outer mitochondrial membrane involves an import pathway via receptors and the general import pore of the TOM complex
    Krimmer, T
    Rapaport, D
    Ryan, MT
    Meisinger, C
    Kassenbrock, CK
    Blachly-Dyson, E
    Forte, M
    Douglas, MG
    Neupert, W
    Nargang, FE
    Pfanner, N
    [J]. JOURNAL OF CELL BIOLOGY, 2001, 152 (02) : 289 - 300
  • [9] The preprotein translocation channel of the outer membrane of mitochondria
    Künkele, KP
    Heins, S
    Dembowski, M
    Nargang, FE
    Benz, R
    Thieffry, M
    Walz, J
    Lill, R
    Nussberger, S
    Neupert, W
    [J]. CELL, 1998, 93 (06) : 1009 - 1019
  • [10] Sam35 of the mitochondrial protein sorting and assembly machinery is a peripheral outer membrane protein essential for cell viability
    Milenkovic, D
    Kozjak, V
    Wiedemann, N
    Lohaus, C
    Meyer, HE
    Guiard, B
    Pfanner, N
    Meisinger, C
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (21) : 22781 - 22785