Screening of transgenic plants by multiplex PCR

被引：25

作者：

Mannerlof, M ^{[1
]}

Tenning, P ^{[1
]}

机构：

[1] DEPT BIOTECHNOL,S-26123 LANDSKRONA,SWEDEN

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1997年 / 15卷 / 01期

关键词：

DNA isolation; multiplex polymerase chain reaction; transgenic plants;

D O I：

10.1007/BF02772111

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A protocol is described, for the rapid screening of a large number of putative transgenic shoots. Genomic DNA is isolated and screened by PCR. To validate the purity of the DNA, PCR amplification is done with primers homologous to an endogenous gene. Multiplex PCR is used to screen for the transgenic shoots with two sets of primers, one set against the endogenous gene (internal control) and the other set against the gene used in transformation. This protocol has been successfully used on maize, melon, oil-seed rape, pepper, petunia, potato, squash, sugar beet and tobacco.

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收藏

页码：38 / 45

页数：8

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