Building of an immunosensor:: How can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?

被引：98

作者：

Briand, Elisabeth

Salmain, Michele

Herry, Jean-Marie

Perrot, Hubert

Compere, Chantal

Pradier, Claire-Marie

机构：

[1] Univ Paris 06, CNRS, UMR 7609, Lab React Surface, F-75252 Paris 05, France

[2] Ecole Natl Super Chim, CNRS, UMR 7576, Lab Chim & Biochim Complexes Mol, F-75231 Paris, France

[3] INRA, Unite Bioadhes & Hyg Mat, F-91300 Massy, France

[4] Lab Interfaces & Syst Electrochim, UPR 15, F-75252 Paris 05, France

[5] IFREMER, Dept Essais & Rech Technol Interface & Capteurs, F-29280 Plouzane, France

来源：

BIOSENSORS & BIOELECTRONICS | 2006年 / 22卷 / 03期

关键词：

mixed SAM's; immunosensor; thiol attachment; IRRAS; QCM;

D O I：

10.1016/j.bios.2006.05.018

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 [生物物理学];

摘要：

Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：440 / 448

页数：9

共 31 条

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[Anonymous], ANALYST

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Evaluation of antibody immobilization methods for piezoelectric biosensor application [J].