68Ga-labelled DOTA-derivatised peptide ligands

被引：190

作者：

Meyer, GJ

Mäcke, H

Schuhmacher, J

Knapp, WH

Hofmann, M

机构：

[1] Hannover Med Sch, Klin Nukl Med, D-30623 Hannover, Germany

[2] Univ Spital Basel, Basel, Switzerland

[3] Deutsch Krebsforschungszentrum, D-6900 Heidelberg, Germany

来源：

EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING | 2004年 / 31卷 / 08期

关键词：

radiopeptides; PET; gallium-68; Ge-68/Ga-68; generator; DOTA;

D O I：

10.1007/s00259-004-1486-0

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Ge-68/Ga-68 generators provide cyclotron-independent access to positron emission tomography (PET) radiopharmaceuticals. We describe a system which allows the safe and efficient handling of Ge-68/Ga-68 generator eluates for labelling of DOTA-derivatised peptide ligands. The system comprises concentration and purification of the Ga-68 eluate as well as labelling and purification steps for peptides, and can be used with different Ge-68/Ga-68 generator types. The suitability and efficiency were tested with two different DOTA-derivatised somatostatin derivatives and a DOTA-derivatised bombesin derivative. Amounts of 10-20 nmol of the peptides were sufficient and resulted in labelling yields of 50% for all peptides. The built-in safety precautions have proven to be appropriate in allowing use of the method for routine clinical applications. The system was set up and operated in a "hot lab" by personnel with no previous experience in the preparation of PET radiopharmaceuticals.

引用

页码：1097 / 1104

页数：8

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