Detection and identification of oxalate-degrading bacteria in human feces

被引:17
作者
Kodama, T [1 ]
Akakura, K [1 ]
Mikami, K [1 ]
Ito, H [1 ]
机构
[1] Chiba Univ, Grad Sch Med, Dept Urol, Chuo Ku, Chiba 2608670, Japan
关键词
calcium-oxalate urolithiasis; chain reaction; human feces; oxalate-degrading bacteria;
D O I
10.1046/j.1442-2042.2002.00488.x
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Background: Oxalate is detoxified (catabolized) via the action of two enzymatic proteins, formyl coenzyme A transferase (encoded by the frc gene) and oxalyl coenzyme A decarboxylase (encoded by the oxc gene), contained in the cytosol of Oxalobacter formigenes that colonizes the human intestinal tract. It is speculated that oxalate-degrading bacteria decrease oxalate absorption from the intestines and their absence in the gastrointestinal tract correlates with the formation of calcium-oxalate urolithiasis. Methods: Two methods of detection and identification of this bacterial strain were studied in human fecal samples collected from Japanese subjects. Genomic DNA was isolated from bacterial culture, and specific 16S rDNA was amplified by polymerase chain reaction (PCR) followed by sequencing. The oxc gene was amplified directly from human feces by PCR using the specific primers. Results: Oxalate-degrading bacteria were identified by comparing the sequences of 16S rDNA. The oxc gene was directly detected from human feces by PCR. It was ascertained that a combined PCR detection method using both 16S rDNA and the oxc gene allows for identification of O. formigenes in human fecal samples. Conclusion: This detection and identification method of oxalate-degrading bacteria using 16S rDNA and oxc gene should be applied in examination of clinical samples.
引用
收藏
页码:392 / 397
页数:6
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