Expression of Helicobacter pylori urease subunit B gene in transgenic rice

被引：28

作者：

Gu, Qing ^{[1
]}

Han, Ning

Liu, Jianyi

Zhu, Muyuan

机构：

[1] Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310012, Peoples R China

[2] Zhejiang Gongshang Univ, Dept Biotechnol, Hangzhou 310035, Peoples R China

[3] Ningbo Municipal Ctr Dis Control & Prevent, Ningbo 315010, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 20期

基金：

中国国家自然科学基金;

关键词：

Helicobacter pylori; transgenic rice; ureB; vaccine;

D O I：

10.1007/s10529-006-9141-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Helicobacter pylori ureB antigen gene was cloned to the 5'-end of gus (beta-glucuronidase) reporter gene between CaMV35S promoter and the octopine synthase (OCS) terminator in the plasmid, pCAMBIA13011. It was then introduced into rice genome by Agrobacterium-mediated transformation. A total of 30 regenerated plants with hygromycin resistance were obtained in the selection media. The putative transgenic individuals were tested for the presence of ureB in the nuclear genome of rice plants by PCR analysis. Expression of ureB gene in rice plants was verified by RT-PCR and Western blot analysis using polyclonal human antiserum for transcription and translation levels respectively. These results provide a basis for further studies on the accumulation level of UreB recombinant protein in transgenic rice and potential utilization of transgenic rice for delivery of edible vaccines against Helicobacter pylori.

引用

页码：1661 / 1666

页数：6

共 16 条

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