On-line electrochemical tagging of cysteines in proteins during nanospray

被引：66

作者：

Rohner, TC

Rossier, JS

Girault, HH ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Chim Mol & Biol, Lab Electrochim Phys & Analyt, CH-1015 Lausanne, Switzerland

[2] DiagnoSwiss SA, CH-1870 Monthey, Switzerland

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2002年 / 4卷 / 09期

关键词：

ESI-MS; nanospray; protein modification;

D O I：

10.1016/S1388-2481(02)00426-5

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

An electrochemical modification of free cysteine residues is studied and characterized by means of quinone addition. Taking advantage of the electrolytic nature of electrospray interfaces (ESI), an electrochemical tagging is performed prior to mass spectrometry (MS) analyses, The tagging has been studied by MS and different mechanisms, involving electrochemical and/or chemical steps, could be characterized. It is demonstrated that the present nanospray is a very efficient tool to obtain cysteine modification, Using the high voltage electrode of the nanospray interface to perform protein specific tagging is a novel method that can be associated to analytical or preparative techniques, such as digestion of proteins or capillary electrophoresis. for post-column modifications. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：695 / 700

页数：6

共 20 条

[1] MECHANISM OF ELECTROSPRAY MASS-SPECTROMETRY - ELECTROSPRAY AS AN ELECTROLYSIS CELL [J].

BLADES, AT ;

IKONOMOU, MG ;

KEBARLE, P .

ANALYTICAL CHEMISTRY, 1991, 63 (19) :2109-2114

[2] Covalent protein adducts of hydroquinone in tissues from rats: Identification and quantitation of sulfhydryl-bound forms [J].

Boatman, RJ ;

English, JC ;

Perry, LG ;

Fiorica, LA .

CHEMICAL RESEARCH IN TOXICOLOGY, 2000, 13 (09) :853-860

[3] Role of quinones in toxicology [J].

Bolton, JL ;

Trush, MA ;

Penning, TM ;

Dryhurst, G ;

Monks, TJ .

CHEMICAL RESEARCH IN TOXICOLOGY, 2000, 13 (03) :135-160

[4] Quantitative analysis of complex protein mixtures using isotope-coded affinity tags [J].

Gygi, SP ;

Rist, B ;

Gerber, SA ;

Turecek, F ;

Gelb, MH ;

Aebersold, R .

NATURE BIOTECHNOLOGY, 1999, 17 (10) :994-999

[5] COVALENT BINDING OF BENZOQUINONE TO REDUCED RIBONUCLEASE - ADDUCT STRUCTURES AND STOICHIOMETRY [J].

HANZLIK, RP ;

HARRIMAN, SP ;

FRAUENHOFF, MM .

CHEMICAL RESEARCH IN TOXICOLOGY, 1994, 7 (02) :177-184

[6]

Herbert B, 2001, ELECTROPHORESIS, V22, P2046, DOI 10.1002/1522-2683(200106)22:10<2046::AID-ELPS2046>3.0.CO

[7]

2-C

[8] Analytical strategies for the detection of sulfide: a review [J].

Lawrence, NS ;

Davis, J ;

Compton, RG .

TALANTA, 2000, 52 (05) :771-784

[9] Characterization of benzoquinone-peptide adducts by electrospray mass spectrometry [J].

Mason, DE ;

Liebler, DC .

CHEMICAL RESEARCH IN TOXICOLOGY, 2000, 13 (10) :976-982

[10] TOXICOLOGY OF QUINONE-THIOETHERS [J].

MONKS, TJ ;

LAU, SS .

CRITICAL REVIEWS IN TOXICOLOGY, 1992, 22 (5-6) :243-270

← 1 2 →