Simultaneous RP-LC determination of additives in soft drinks

被引：52

作者：

Dossi, N. ^{[1
]}

Toniolo, R. ^{[1
]}

Susmel, S. ^{[1
]}

Pizzariello, A. ^{[1
]}

Bontempelli, G. ^{[1
]}

机构：

[1] Univ Udine, Dept Chem Sci & Technol, I-33100 Udine, Italy

来源：

CHROMATOGRAPHIA | 2006年 / 63卷 / 11-12期

关键词：

column liquid chromatography; sweeteners; preservatives and dyes; food analysis; soft drinks;

D O I：

10.1365/s10337-006-0793-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reverse-phase HPLC method for the simultaneous determination of the main artificial sweeteners, preservatives and dyes present in soft drinks is proposed. It involves the use of a 10 mu m LiChrosorb RP18 column and a binary eluent consisting of aqueous 0.1 M phosphate buffer (pH 4.0) added with methanol, according to a suitable gradient elution program. Good separations were obtained within less than 20-min run-time, with a satisfactory precision. The sensitivity of spectrophotometric detection was optimised by adopting a wavelength switching technique, thus achieving for all the additives considered detection limits ranging from 0.1 to 3.0 mg L-1, well below the maximum permitted levels. The method was applied to some commercial soft drinks, whose analysis required minimum pre-treatment before direct injection.

引用

页码：557 / 562

页数：6

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