Solution phase synthesis of dithymidine phosphorothioate by a phosphotriester method using new S-protecting groups

被引：6

作者：

Puschl, A ^{[1
]}

Kehler, J ^{[1
]}

Dahl, O ^{[1
]}

机构：

[1] UNIV COPENHAGEN,HC ORSTED INST,DEPT CHEM,DK-2100 COPENHAGEN,DENMARK

来源：

NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 1-2期

关键词：

D O I：

10.1080/07328319708002529

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A phosphotriester method for the synthesis of dithymidine phosphorothioates with eight S-protecting groups has been investigated. Three of the S-protecting groups possesed catalytic activity, however side reactions occurred under deprotection. The best S-protecting group was 4-chloro-2-nitrobenzyl which could be removed with a minimum of side reactions (0.3%). The Coupling reagent PyFNOP (11) gave protected dithymidine phosphorothioate in 96% yield after 15 min coupling.

引用

页码：145 / 158

页数：14

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