FtsZ Filament Dynamics at Steady State: Subunit Exchange with and without Nucleotide Hydrolysis

We have measured three aspects of FtsZ filament dynamics at steady state: rates of GTP hydrolysis, Subunit exchange between protofilaments, and disassembly induced by dilution or excess GDP. All three reactions were slowed with an increase in the potassium concentration front 100 to 500 mM, via replacement of potassium with rubidium, or with an increase in the magnesium concentration from 5 to 20 mM. Electron microscopy showed that the polymers assembled Under the conditions of fastest assembly were predominantly short, one-stranded protofilaments, whereas under conditions of slower dynamics, the protofilaments tended to associate into long, thin bundles. We Suggest that exchange of subunits between protofilaments at steady state Involves two separate mechanisms: (1) fragmentation or dissociation of Subunits from protofilament ends following GTP hydrolysis and (2) reversible association and dissociation of subunits from protofilament ends independent of hydrolysis. Exchange of nucleotides oil these recycling subunits Could give the appearance of exchange directly into the polymer. Several of our suggest that exchange of nucleotide Call take place oil these recycling subunits, but not directly into the FtsZ polymer. Annealing of protofilaments was demonstrated for the L68W mutant in EDTA buffer but not in Mg buffer, where rapid cycling Of Subunits may obscure the effect of annealing. We also reinvestigated the nucleotide composition of FtsZ polymers at steady state. We found that the GDP:GTP ratio was 50:50 for concentrations of GTP > 100 mu M, significantly higher than the 20:80 ratio previously reported at 20 mu M GTP.