Salmonella enteritidis agfBAC operon encoding thin, aggregative fimbriae

被引:108
作者
Collinson, SK
Clouthier, SC
Doran, JL
Banser, PA
Kay, WW
机构
[1] UNIV VICTORIA,DEPT BIOCHEM & MICROBIOL,VICTORIA,BC V8W 3P6,CANADA
[2] UNIV VICTORIA,CANADIAN BACTERIAL DIS NETWORK,VICTORIA,BC V8W 3P6,CANADA
[3] MICROTEK INT LTD,SAANICHTON,BC V8X 3X1,CANADA
关键词
D O I
10.1128/jb.178.3.662-667.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Salmonella enteritidis produces thin, aggregative fimbriae, named SEF17, which are composed of polymerized AgfA fimbrin proteins. DNA sequence analysis of a 2-kb region of S. enteritidis DNA revealed three contiguous genes, agfBAC. The 453-bp agfA gene encodes the AgfA fimbrin, which was predicted to be 74% identical and 86% similar in primary sequence to the Escherichia coli curli structural protein, CsgA. pH;IG, a pUC18 derivative containing a 3.0-kb HindIII fragment encoding agfBAC, directed the in vitro expression of the major AgfA fimbrin, with an M(r) of 17,000, and a minor AgfB protein, with an M(r) of 16,000, encoded by the 453-bp agfB gene. AgfA was not expressed from pDAG, a pUC18 derivative containing a 3.1-kb DraI DNA fragment encoding agfA but not agfB. Primer extension analysis identified two adjacent transcription start sites located immediately upstream of agfB in positions analogous to those of the E. coli curlin csgBA operon. No transcription start sites were located immediately upstream of agfA or agfC. Northern (RNA) blot analysis confirmed that transcription of agfA was initiated from the agfB promoter region. Secondary-structure analysis of the putative mRNA transcript for agfBAC predicted the formation of a stem-loop structure (Delta G(o), -22 kcal/mol [-91 kJ/mol]) in the intercistronic region between agfA and agfC, which may be involved in stabilization of the agfBA portion of the agfBAC transcript. agfBAC and flanking regions had a high degree of sequence similarity with those counterparts of the E. coli curlin csgBA region for which sequence data are available. These data are demonstrative of the high degree of similarity between S. enteritidis SEF17 fimbriae and E. coli curli with respect to fimbrin amino acid sequence and genetic organization and, therefore, are indicative of a common and relatively recent ancestry.
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页码:662 / 667
页数:6
相关论文
共 31 条
[1]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[2]  
ARNQVIST A, 1992, MOL MICROBIOL, V6, P2443
[3]   SIGMA(S)-DEPENDENT GROWTH-PHASE INDUCTION OF THE CSGBA PROMOTER IN ESCHERICHIA-COLI CAN BE ACHIEVED IN-VIVO BY SIGMA(70) IN THE ABSENCE OF THE NUCLEOID-ASSOCIATED PROTEIN H-NS [J].
ARNQVIST, A ;
OLSEN, A ;
NORMARK, S .
MOLECULAR MICROBIOLOGY, 1994, 13 (06) :1021-1032
[4]   PROCESSED MESSENGER-RNA WITH DIFFERENTIAL STABILITY IN THE REGULATION OF ESCHERICHIA-COLI PILIN GENE-EXPRESSION [J].
BAGA, M ;
GORANSSON, M ;
NORMARK, S ;
UHLIN, BE .
CELL, 1988, 52 (02) :197-206
[5]  
BAUMLER AJ, 1995, J BACTERIOL, V177, P2087
[6]   MECHANISMS OF MESSENGER-RNA DECAY IN BACTERIA - A PERSPECTIVE [J].
BELASCO, JG ;
HIGGINS, CF .
GENE, 1988, 72 (1-2) :15-23
[7]   MESSENGER-RNA PROCESSING INDEPENDENT OF RNASE-III AND RNASE-E IN THE EXPRESSION OF THE F1845 FIMBRIAL ADHESIN OF ESCHERICHIA-COLI [J].
BILGE, SS ;
APOSTOL, JM ;
ALDAPE, MA ;
MOSELEY, SL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (04) :1455-1459
[8]   AN INTERCISTRONIC STEM-LOOP STRUCTURE FUNCTIONS AS AN MESSENGER-RNA DECAY TERMINATOR NECESSARY BUT INSUFFICIENT FOR PUF MESSENGER-RNA STABILITY [J].
CHEN, CYA ;
BEATTY, JT ;
COHEN, SN ;
BELASCO, JG .
CELL, 1988, 52 (04) :609-619
[9]   CHARACTERIZATION OF 3 FIMBRIAL GENES, SEFABC, OF SALMONELLA-ENTERITIDIS [J].
CLOUTHIER, SC ;
MULLER, KH ;
DORAN, JL ;
COLLINSON, SK ;
KAY, WW .
JOURNAL OF BACTERIOLOGY, 1993, 175 (09) :2523-2533
[10]   PURIFICATION AND CHARACTERIZATION OF THIN, AGGREGATIVE FIMBRIAE FROM SALMONELLA-ENTERITIDIS [J].
COLLINSON, SK ;
EMODY, L ;
MULLER, KH ;
TRUST, TJ ;
KAY, WW .
JOURNAL OF BACTERIOLOGY, 1991, 173 (15) :4773-4781