Membrane-assembly of carbon microelectrodes for the electrochemical study of redox proteins

被引：7

作者：

Bianco, P

Lattuca, C

机构：

[1] B.I.P., C.N.R.S., 13402 Marseille Cedex 20

来源：

ANALYTICA CHIMICA ACTA | 1997年 / 353卷 / 01期

关键词：

membrane-microelectrode; c-type cytochromes; ferredoxins;

D O I：

10.1016/S0003-2670(97)00384-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel membrane-assembly of microelectrodes has been used to study the electrochemistry of several redox proteins (c-type cytochromes, ferredoxins). The solutions of protein (5 mu L) were entrapped between a dialysis membrane and the assembly surface. Near-steady-state current-voltage curves were obtained; by combining cyclic and square-wave voltammetry, a detection limit of about 20 pmol of analyte was attained. In addition, it has been demonstrated that direct electron-transfer was observed for cytochrome c and ferredoxins in the presence of a promoter within the electrochemical cell. Low molar-mass agents were able to diffuse through the membrane to develop their promoting effect. (C) 1997 Elsevier Science B.V.

引用

页码：53 / 59

页数：7

共 26 条

[1]

ARMSTRONG FA, 1990, STRUCT BOND, V72, P137

[2] ELECTROCHEMICAL INVESTIGATIONS OF CYTOCHROME C-3 FROM DESULFOVIBRIO-DESULFURICANS NORWAY AT SOLID ELECTRODES [J].