Isolation and characterisation of acid-solubilised collagen from the skin of Nile tilapia (Oreochromis niloticus)

被引：115

作者：

Zeng, Shao-kui ^{[2
]}

Zhang, Chao-hua ^{[2
]}

Lin, Hong ^{[1
]}

Yang, Ping ^{[2
]}

Hong, Peng-zhi ^{[2
]}

Jiang, Zhihong ^{[2
]}

机构：

[1] Ocean Univ China, Fac Food Sci & Technol, Qingdao 266033, Shandong, Peoples R China

[2] GuangDong Ocean Univ, Fac Food Technol, Zhanjiang 524025, Guangdong, Peoples R China

来源：

FOOD CHEMISTRY | 2009年 / 116卷 / 04期

基金：

广东省科技计划;

关键词：

Collagen; Oreochromis niloticus; Skin; Acid-solubilised collagen (ASC); Solubility; alpha Chain; I COLLAGEN; FISH; MUSCLE;

D O I：

10.1016/j.foodchem.2009.03.038

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Acid-solubilised collagen (ASC) was extracted from the skin of Nile tilapia (Oreochromis niloticus) and characterisation was studied. The results indicated that the yield of ASC was 39.4% on the basis of dry weight. This ASC was rich in glycine (35.6%). The amount of imino acids, proline and hydroxyproline, in ASC was 210 residues per 1000 residues. The ultraviolet (UV) absorption spectrum of ASC showed that the distinct absorption was at 220 run. ASC showed transition curve at maximum temperature of 32.0 degrees C in 0.05 M acetic acid, about 12 degrees C lower than that of calf skin collagen. Maximum solubility (0.75 mg/ml) in 0.5 M acetic acid was observed at pH 3. Solubility reached the minimum at pH 7. A sharp decrease in Solubility was observed in 2% (w/v) NaCl or above. Biochemical Studies indicated that ASC was composed of the alpha 1 alpha 2 alpha 3 heterotrimers. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：879 / 883

页数：5

共 18 条

[1] Fourier-transform Raman spectroscopy of ivory .2. Spectroscopic analysis and assignments [J].

Edwards, HGM ;

Farwell, DW ;

Holder, JM ;

Lawson, EE .

JOURNAL OF MOLECULAR STRUCTURE, 1997, 435 (01) :49-58

[2]

Foegeding E.A., 1996, Food Chemistry, P879

[3] Structural aspects of fish skin collagen which forms ordered arrays via liquid crystalline states [J].

Giraud-Guille, MM ;

Besseau, L ;

Chopin, C ;

Durand, P ;

Herbage, D .

BIOMATERIALS, 2000, 21 (09) :899-906

[4]

Helcke T., 2000, INT FOOD INGREDIENTS, V1, P6

[5] Isolation and characterisation of acid and pepsin-solubilised collagens from the skin of Brownstripe red snapper (Lutjanus vitta) [J].

Jonglareonrak, A ;

Benjakul, S ;

Visessanguan, W ;

Nagai, T ;

Tanaka, M .

FOOD CHEMISTRY, 2005, 93 (03) :475-484

[6] FISH SKIN TYPE-I COLLAGEN - WIDE DISTRIBUTION OF AN ALPHA-3 SUBUNIT IN TELEOSTS [J].

KIMURA, S ;

OHNO, Y ;

MIYAUCHI, Y ;

UCHIDA, N .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1987, 88 (01) :27-34

[7] CHARACTERIZATION OF FISH MUSCLE TYPE-I COLLAGEN [J].

KIMURA, S ;

ZHU, XP ;

MATSUI, R ;

SHIJOH, M ;

TAKAMIZAWA, S .

JOURNAL OF FOOD SCIENCE, 1988, 53 (05) :1315-1318

[8] CONNECTIVE TISSUES AND COLLAGENS OF COD DURING STARVATION [J].

LOVE, RM ;

YAMAGUCHI, K ;

CREACH, Y ;

LAVETY, J .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1976, 55 (04) :487-492

[9]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[10] CHARACTERIZATION OF HAKE (MERLUCCIUS-MERLUCCIUS L) AND TROUT (SALMO-IRIDEUS GIBB) COLLAGEN [J].

MONTERO, P ;

BORDERIAS, J ;

TURNAY, J ;

LEYZARBE, MA .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1990, 38 (03) :604-609

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