Sustained release of recombinant human growth hormone from dextran via hydrolysis of an imine bond

被引：14

作者：

Battersby, J ^{[1
]}

Clark, R ^{[1
]}

Hancock, W ^{[1
]}

PuchuluCampanella, E ^{[1
]}

Haggarty, N ^{[1
]}

Poll, D ^{[1
]}

Harding, D ^{[1
]}

机构：

[1] MASSEY UNIV,DEPT CHEM & BIOCHEM,SEPARAT SCI PROGRAMME,PALMERSTON NORTH,NEW ZEALAND

来源：

JOURNAL OF CONTROLLED RELEASE | 1996年 / 42卷 / 02期

关键词：

sustained release; human growth hormone; dextran; imine conjugate;

D O I：

10.1016/0168-3659(96)01370-3

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Dextran T40 was oxidized with periodate to form aldehyde groups to which recombinant human growth hormone (rhGH) was attached. Complex formation involved the reversible formation of an imine conjugate and the extent of complex formation was proportional to the degree of oxidation of the dextran. The complex was characterized by a variety of techniques including SEC, SDS-PAGE and IEF. In vitro, the rate of release of rhGH from a rhGH-dextran complex was inversely proportional to the degree of oxidation of the dextran. The released protein was characterized by peptide mapping, N-terminal sequencing, SEC and SDS-PAGE. Sustained release of rhGH was observed in vivo, based on the observation of significant weight gain in a rat bioassay.

引用

页码：143 / 156

页数：14

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