Sialyl Lewis x liposomes as a multivalent ligand and inhibitor of E-selectin mediated cellular adhesion

被引：101

作者：

DeFrees, SA ^{[1
]}

Phillips, L ^{[1
]}

Guo, L ^{[1
]}

Zalipsky, S ^{[1
]}

机构：

[1] SEQUUS PHARMACEUT INC,MENLO PK,CA 94025

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1996年 / 118卷 / 26期

关键词：

D O I：

10.1021/ja954122g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A sialyl Lewis x-PEG-DSPE derivative (3) has been prepared using a combined chemical enzymatic approach and incorporated into mPEG-DSPE containing liposomes (PEG, poly(ethylene glycol); mPEG, methoxypoly(ethylene glycol); DSPE, distearoylphosphatidylethanolamine). Several liposomal formulations of 3 were evaluated as inhibitors of E-selectin mediated cellular adhesion in an ELISA assay and were found to be similar to 750-fold more potent than the nonliposomal oligosaccharide (2) and greater than 5000-fold more potent than the natural glycotope structure (1). The dramatic increase in potency of the liposomal formulations suggests that oligosaccharide multivalency enhances inhibition of E-selectin adhesion and is an effective approach to the design of more potent selectin cell adhesion inhibitors.

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页码：6101 / 6104

页数：4

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