Detection of Helicobacter pylori in water by direct PCR

Aims: This paper demonstrates a rapid, simple method for the detection of Helicobacter pylori in water that eliminates the need for recovery of cells or DNA extraction prior to PCR. Methods and Results: Direct polymerase chain reaction (DPCR) with primers specific for H. pylori ureA (urease, subunit A) were used to detect H. pylori added to groundwater. DPCR also detected H. pylori in a naturally contaminated water sample. Conclusions: DPCR should provide an improved method to assess contamination of water by H. pylori. Significance and Impact of the Study: This simple, rapid method for detection of H. pylori in water will provide an improved means to investigate the possible role of water as a disease vector.