Cell-selective metabolic labeling of proteins

被引：140

作者：

Ngo, John T. ^{[1
]}

Champion, Julie A. ^{[1
]}

Mahdavi, Alborz ^{[1
]}

Tanrikulu, I. Caglar ^{[1
]}

Beatty, Kimberly E. ^{[1
]}

Connor, Rebecca E. ^{[1
]}

Yoo, Tae Hyeon ^{[1
]}

Dieterich, Daniela C. ^{[2
]}

Schuman, Erin M. ^{[2
]}

Tirrell, David A. ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

[2] CALTECH, Div Biol, Pasadena, CA 91125 USA

来源：

NATURE CHEMICAL BIOLOGY | 2009年 / 5卷 / 10期

基金：

美国国家卫生研究院;

关键词：

NEWLY SYNTHESIZED PROTEINS; NONCANONICAL AMINO-ACIDS; MAMMALIAN-CELLS; BACTERIAL-CELLS; IDENTIFICATION; PROTEOMICS; CHEMISTRY;

D O I：

10.1038/nchembio.200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.

引用

页码：715 / 717

页数：3

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