Characterisation of six α-expansin genes in Gossypium hirsutum (upland cotton)

被引：94

作者：

Harmer, SE ^{[1
]}

Orford, SJ ^{[1
]}

Timmis, JN ^{[1
]}

机构：

[1] Univ Adelaide, Dept Mol Biosci, Discipline Genet, Adelaide, SA 5005, Australia

来源：

MOLECULAR GENETICS AND GENOMICS | 2002年 / 268卷 / 01期

基金：

澳大利亚研究理事会;

关键词：

expansin; Gossypium hirsutum; cotton fibre; RT-PCR; gene family;

D O I：

10.1007/s00438-002-0721-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A genomic library screen and PCR-based strategies were employed to isolate six genes with sequence similarity to a cotton fibre-specific mRNA encoding an alpha-expansin. alpha-Expansins are cell wall proteins that facilitate cell wall extension by disruption of non-covalent bonds between wall components. The characterisation and expression analysis of these six novel genes (GhExp1-GhExp6) is described. Four of them (GhExp3-GhExp6) are expressed within multiple tissues of the cotton plant and two (GhExp1 and GhExp2) give rise to transcripts that are specific to the developing cotton fibre. GhExp1 transcripts are highly abundant in the fibre, while transcripts for GhExp2 are detected at a low level. Cotton fibres are highly elongated cells of the ovule epidermis, and we envisage that GhExp1 may play an important role in cell wall extension during development.

引用

页码：1 / 9

页数：9

共 48 条

[1] DEVELOPMENT OF THE COTTON FIBER [J].

BASRA, AS ;

MALIK, CP .

INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1984, 89 :65-113

[2] A CATALOG OF SPLICE JUNCTION AND PUTATIVE BRANCH POINT SEQUENCES FROM PLANT INTRONS [J].

BROWN, JWS .

NUCLEIC ACIDS RESEARCH, 1986, 14 (24) :9549-9559

[3] Modification of expansin protein abundance in tomato fruit alters softening and cell wall polymer metabolism during ripening [J].

Brummell, DA ;

Harpster, MH ;

Civello, PM ;

Palys, JM ;

Bennett, AB ;

Dunsmuir, P .

PLANT CELL, 1999, 11 (11) :2203-2216

[4] Auxin-regulated genes encoding cell wall-modifying proteins are expressed during early tomato fruit growth [J].

Catalá, C ;

Rose, JKC ;

Bennett, AB .

PLANT PHYSIOLOGY, 2000, 122 (02) :527-534

[5] Expression of an expansin is associated with endosperm weakening during tomato seed germination [J].

Chen, F ;

Bradford, KJ .

PLANT PHYSIOLOGY, 2000, 124 (03) :1265-1274

[6] Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana [J].

Cho, HT ;

Cosgrove, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (17) :9783-9788

[7] Expansins in deepwater rice internodes [J].

Cho, HT ;

Kende, H .

PLANT PHYSIOLOGY, 1997, 113 (04) :1137-1143

[8] Expression of expansin genes is correlated with growth in deepwater rice [J].

Cho, HT ;

Kende, H .

PLANT CELL, 1997, 9 (09) :1661-1671

[9] An expansin gene expressed in ripening strawberry fruit [J].

Civello, PM ;

Powell, ALT ;

Sabehat, A ;

Bennett, AB .

PLANT PHYSIOLOGY, 1999, 121 (04) :1273-1279

[10] BIOPHYSICAL CONTROL OF PLANT-CELL GROWTH [J].

COSGROVE, D .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1986, 37 :377-405

← 1 2 3 4 5 →