Synthesis and biological evaluation of a cross-linked hyaluronan-mitomycin C hydrogel

被引：47

作者：

Li, H ^{[1
]}

Liu, YC ^{[1
]}

Shu, XZ ^{[1
]}

Gray, SD ^{[1
]}

Prestwich, GD ^{[1
]}

机构：

[1] Univ Utah, Dept Med Chem, Salt Lake City, UT 84108 USA

来源：

BIOMACROMOLECULES | 2004年 / 5卷 / 03期

关键词：

D O I：

10.1021/bm034463j

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cross-linked hyaluronan (HA) hydrogel that contained a covalently bound derivative of the anti-proliferative drug mitomycin C (MMC) was synthesized and evaluated in vitro and in vivo. The HA-MMC hydrogel was prepared by coupling MMC-aziridinyl-N-acrylate with thiol-modified HA followed by cross-linking C, with poly(ethylene glycol) diacrylate (PEGDA). MMC was released from 0.5% and 2.0% MMC films by hydrolysis in proportion to the MMC loading. When incubated in vitro with human T31 tracheal scar fibroblasts, 0.5% MMC films inhibited proliferation, whereas 2.0% MMC films were cytotoxic. When implanted in vivo into a rat peritoneal cavity, neither 0.5% nor 2.0% HA-MMC films elicited a severe peritoneal fluid leukocyte response. Importantly, MMC reduced the thickness of fibrous tissue formed surrounding the implanted films. Thus, cross-linked HA-MMC films have strong potential as anti-fibrotic barriers for the prevention of post-surgical adhesions.

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页码：895 / 902

页数：8

相关论文

共 43 条

[1] Evaluation of antitumor activities of hyaluronate binding antitumor drugs: Synthesis, characterization and antitumor activity [J].

Akima, K ;

Ito, H ;

Iwata, Y ;

Matsuo, K ;

Watari, N ;

Yanagi, M ;

Hagi, H ;

Oshima, K ;

Yagita, A ;

Atomi, Y ;

Tatekawa, I .

JOURNAL OF DRUG TARGETING, 1996, 4 (01) :1-&

[2] Prevention of postsurgical adhesions with an autocrosslinked hyaluronan derivative gel [J].

Belluco, C ;

Meggiolaro, F ;

Pressato, D ;

Pavesio, A ;

Bigon, E ;

Donà, M ;

Forlin, M ;

Nitti, D ;

Lise, M .

JOURNAL OF SURGICAL RESEARCH, 2001, 100 (02) :217-221

[3] CHARACTERIZATION OF THE CELLULAR REDUCTION OF 3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) - SUBCELLULAR-LOCALIZATION, SUBSTRATE DEPENDENCE, AND INVOLVEMENT OF MITOCHONDRIAL ELECTRON-TRANSPORT IN MTT REDUCTION [J].

BERRIDGE, MV ;

TAN, AS .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 303 (02) :474-482

[4] Prevention of tissue injury and postsurgical adhesions by precoating tissues with hyaluronic acid solutions [J].

Burns, JM ;

Skinner, K ;

Colt, J ;

Sheidlin, A ;

Bronson, R ;

Yaacobi, Y ;

Goldberg, EP .

JOURNAL OF SURGICAL RESEARCH, 1995, 59 (06) :644-652

[5]

Burns JW, 1997, EUR J SURG, V163, P40

[6] Semisynthetic resorbable materials from hyaluronan esterification [J].

Campoccia, D ;

Doherty, P ;

Radice, M ;

Brun, P ;

Abatangelo, G ;

Williams, DF .

BIOMATERIALS, 1998, 19 (23) :2101-2127

[7] Mitomycin C for the prevention of adhesion formation after endoscopic sinus surgery: A randomized, controlled study [J].

Chung, JH ;

Cosenza, MJ ;

Rahbar, R ;

Metson, RB .

OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 2002, 126 (05) :468-474

[8] MITOMYCIN-C - REVIEW [J].

CROOKE, ST ;

BRADNER, WT .

CANCER TREATMENT REVIEWS, 1976, 3 (03) :121-139

[9] Mitomycin-C prevents reformation of intra-abdominal adhesions after adhesiolysis [J].

Çubukçu, A ;

Alponat, A ;

Gönüllü, NN .

SURGERY, 2002, 131 (01) :81-84

[10] An experimental study evaluating the effect of mitomycin C on the prevention of postoperative intraabdominal adhesions [J].

Çubukçu, A ;

Alponat, A ;

Gönüllü, NN ;

Özkan, S ;

Erçin, C .

JOURNAL OF SURGICAL RESEARCH, 2001, 96 (02) :163-166

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