Using FM1-43 to study neuropeptide granule dynamics and exocytosis

被引：82

作者：

Brumback, AC

Lieber, JL

Angleson, JK

Betz, WJ

机构：

[1] Univ Colorado, Hlth Sci Ctr, Dept Physiol & Biophys, Denver, CO 80262 USA

[2] Univ Colorado, Hlth Sci Ctr, Med Sci Training Program, Denver, CO 80262 USA

[3] Univ Denver, Dept Sci Biol, Denver, CO 80208 USA

来源：

METHODS | 2004年 / 33卷 / 04期

关键词：

D O I：

10.1016/j.ymeth.2004.01.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the study of neuropeptide secretion and membrane trafficking, the fluorescent dye FM1-43 provides the ability to label selectively those structures that are undergoing exocytosis and endocytosis in living cells in real time. This review describes the unique properties of the FM dyes that make them ideal for studying neuropeptide granule dynamics and discusses various techniques that take advantage of FM dyes. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：287 / 294

页数：8

共 24 条

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