Mitochondria-Immobilized pH-Sensitive Off-On Fluorescent Probe

被引：512

作者：

Lee, Min Hee ^{[1
]}

Park, Nayoung ^{[2
]}

Yi, Chunsik ^{[3
]}

Han, Ji Hye ^{[3
]}

Hong, Ji Hye ^{[4
]}

Kim, Kwang Pyo ^{[4
]}

Kang, Dong Hoon ^{[5
,6
]}

Sessler, Jonathan L. ^{[1
]}

Kang, Chulhun ^{[3
]}

Kim, Jong Seung ^{[2
]}

机构：

[1] Univ Texas Austin, Dept Chem, Austin, TX 78712 USA

[2] Korea Univ, Dept Chem, Seoul 136701, South Korea

[3] Kyung Hee Univ, Sch East West Med Sci, Yongin 446701, South Korea

[4] Kyung Hee Univ, Dept Appl Chem, Coll Appl Sci, Yongin 446701, South Korea

[5] Ewha Womans Univ, Dept Life Sci, Seoul 120750, South Korea

[6] Ewha Womans Univ, Res Ctr Cell Homeostasis, Seoul 120750, South Korea

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2014年 / 136卷 / 40期

基金：

新加坡国家研究基金会; 美国国家卫生研究院;

关键词：

OXIDATIVE-PHOSPHORYLATION; HEART-MITOCHONDRIA; LIVING CELLS; CALCIUM; PROTEIN; MITOPHAGY; MEMBRANE;

D O I：

10.1021/ja506301n

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

We report here a mitochondria-targetable pH-sensitive probe that allows for a quantitative measurement of mitochondrial pH changes, as well as the real-time monitoring of pH-related physiological effects in live cells. This system consists of a piperazine-linked naphthalimide as a fluorescence off-on signaling unit, a cationic triphenylphosphonium group for mitochondrial targeting, and a reactive benzyl chloride subunit for mitochondrial fixation. It operates well in a mitochondrial environment within whole cells and displays a desirable off-on fluorescence response to mitochondrial acidification. Moreover, this probe allows for the monitoring of impaired mitochondria undergoing mitophagic elimination as the result of nutrient starvation. It thus allows for the monitoring of the organelle-specific dynamics associated with the conversion between physiological and pathological states.

引用

页码：14136 / 14142

页数：7

共 44 条

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Mitochondrial pH monitored by a new engineered green fluorescent protein mutant [J].