EPR examination of Zn2+ and Cu2+ binding by pigmented soil fungi Cladosporium cladosporioides

被引:49
作者
Buszman, Ewa
Pilawa, Barbara
Zdybel, Magdalena
Wilczynski, Slawomir
Gondzik, Anna
Witoszynska, Teresa
Wilczok, Tadeusz
机构
[1] Med Univ Silesia, Sch Pharm, Dept Pharmaceut Chem, PL-41200 Sosnowiec, Poland
[2] Med Univ Silesia, Sch Pharm, Dept Phys Med, PL-41200 Sosnowiec, Poland
[3] Med Univ Silesia, Sch Pharm, Dept Mol Biol & Genet, PL-41200 Sosnowiec, Poland
关键词
Cladosporium cladosporioides; melanin; Zn2+; Cu2+; EPR; free radicals;
D O I
10.1016/j.scitotenv.2005.05.028
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The purpose of this study was to examine the usefulness of electron paramagnetic resonance spectroscopy (EPR) to estimate zinc and copper ions biosorption from the environment by pigmented soil fungi Cladosporium cladosporioides. The existence of a low amount of pheornelanin, besides eumelanin, in C cladosporioides samples was proved by the analysis of shape of their EPR spectra. Concentration of o-semiquinone free radicals in crude mycelium was 2.4 x 10(17) spin/g. Changes in free radicals system of C cladosporioides cultured in the presence of Zn2+ and Cu2+ were analysed. Both magnetic and chemical interactions of zinc and copper ions with free radicals in C cladosporioides melanin were found. Magnetically interacting diamagnetic Zn2+ ions increased the concentration of o-semiquinone free radicals in melanin existing in C cladosporioides mycelium, whereas paramagnetic Cu2+ ions decreased this concentration. Chemical interactions of Zn2+ and Cu2+ ions decreased the free radical concentrations in C cladosporioides melanin. Homogeneously distributed free radicals in C cladosporioides melanin rise its activity in biosorption processes. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:195 / 205
页数:11
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