Codon optimization improves heterologous expression of a Schistosoma mansoni cDNA in HEK293 cells

被引：38

作者：

Hamdan, FF ^{[1
]}

Mousa, A ^{[1
]}

Ribeiro, P ^{[1
]}

机构：

[1] McGill Univ, Inst Parasitol, Ste Anne De Bellevue, PQ H9X 3V9, Canada

来源：

PARASITOLOGY RESEARCH | 2002年 / 88卷 / 06期

基金：

加拿大健康研究院;

关键词：

D O I：

10.1007/s00436-001-0585-0

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Differences in codon usage can seriously hamper the expression of cloned cDNAs in heterologous systems. In this study, we show that the expression of a cloned Schistosoma mansoni cDNA in cultured HEK293 cells was dramatically increased by rewriting a portion of the cDNA according to human preferred codon usage, suggesting that codon optimization is a valuable strategy for improving the heterologous expression of helminth sequences. We further describe a simple modification of a recursive PCR-based method, which allows the rewriting of long stretches of DNA sequence in a single PCR reaction. This method can be used to optimize the codon usage of virtually any DNA from helminths and other parasites.

引用

页码：583 / 586

页数：4

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