Ceramide induces apoptosis in PC12 cells

被引：100

作者：

Hartfield, PJ

Mayne, GC

Murray, AW

机构：

[1] School of Biological Sciences, Flinders Univ. of South Australia, Adelaide, SA 5001

来源：

FEBS LETTERS | 1997年 / 401卷 / 2-3期

基金：

英国医学研究理事会;

关键词：

ceramide; apoptosis; PC12; cell; interleukin-1 beta converting enzyme-like protease;

D O I：

10.1016/S0014-5793(96)01460-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The novel lipid second messenger, ceramide, induced apoptosis in PC12 cells as determined morphologically by nuclear appearance and internucleosomal DNA fragmentation, Apoptosis was induced by exogenous C-2-ceramide in a dose- and time-dependent manner, Natural ceramide and C-6-ceramide had a similar effect, This response mas specific since the structural analog C-2-dihydroceramide and other related lipids failed to initiate apoptosis, The apoptotic effect of ceramide also depends critically on cell plating density, Furthermore, the peptide inhibitor of interleukin-1 beta converting enzyme (ICE)-like proteases, Z-VAD.FMK, completely prevented the nuclear changes induced by ceramide, implicating the involvement of ICE-like protease activation in ceramide-induced apoptosis in PC12 cells.

引用

页码：148 / 152

页数：5

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