Isolation, purification and partial characterization of sulphite oxidase from Malva sylvestris

被引：14

作者：

Ganai, BA ^{[1
]}

Masood, A ^{[1
]}

Baig, MA ^{[1
]}

机构：

[1] ALL INDIA INST MED SCI, DEPT BIOCHEM, NEW DELHI 110029, INDIA

来源：

PHYTOCHEMISTRY | 1997年 / 45卷 / 05期

关键词：

Malva sylvestris; Malvaceae; sulphite oxidase; sulphite;

D O I：

10.1016/S0031-9422(97)82709-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sulphite oxidase (EC 1.8.3.1) was isolated from Malva sylvestris leaves and partially characterized. The enzyme was homogeneous with respect to its size and charge, as judged by gel filtration and PAGE. The M-r of the enzyme as estimated by gel filtration was 27 000. The values of Stokes' radius and frictional ratio were 2.29 nm and 1.16, respectively, and the K-m was 5.33 x 10(-3) M. (C) 1997 Elsevier Science Ltd. All rights reserved.

引用

页码：879 / 880

页数：2

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