Regulation of fowl sperm flagellar motility by protein phosphatase type 1 and its relationship with dephosphorylation of axonemal and/or accessory cytoskeletal proteins

被引:20
作者
Ashizawa, K
Hashimoto, K
Tsuzuki, Y
机构
[1] Laboratory of Animal Reproduction, Faculty of Agriculture, Miyazaki University
关键词
D O I
10.1006/bbrc.1997.6738
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The motility of demembranated fowl spermatozoa was vigorous at 30 degrees C in the presence of ATP, but decreased markedly following the addition of recombinant protein phosphatase type 1 (PP-1) supplemented with Mn2+. This inhibition was not restored by the addition of cAMP, within the range 1-1000 mu M, but instantly restored by the addition of 50 ng/ml trypsin. Phosphorylation of demembranated fowl sperm proteins during incubation with [gamma-P-32]ATP at 30 degrees C was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A marked difference in phosphorylation status was observed in approximately 116, 86, 79, 50 and 29-kDa proteins. These proteins were dephosphorylated in the presence of PP-1 and Mn2+ compared with those in control samples. These results suggest that PP-1-mediated dephosphorylation of some of these proteins of the axoneme and/or accessory cytoskeletal components of fowl spermatozoa may be involved in the inhibition of motility. (C) 1997 Academic Press.
引用
收藏
页码:108 / 112
页数:5
相关论文
共 35 条
  • [1] REGULATION OF HUMAN SPERM MOTILITY AND HYPERACTIVATION COMPONENTS BY CALCIUM, CALMODULIN, AND PROTEIN PHOSPHATASES
    AHMAD, K
    BRACHO, GE
    WOLF, DP
    TASH, JS
    [J]. ARCHIVES OF ANDROLOGY, 1995, 35 (03): : 187 - 208
  • [2] ASHIZAWA K, 1993, J REPROD FERTIL, V99, P415, DOI 10.1530/jrf.0.0990415
  • [3] ASHIZAWA K, 1989, J REPROD FERTIL, V86, P271, DOI 10.1530/jrf.0.0860271
  • [4] PRESENCE OF PROTEIN PHOSPHATASE TYPE-1 AND ITS INVOLVEMENT IN TEMPERATURE-DEPENDENT FLAGELLAR MOVEMENT OF FOWL SPERMATOZOA
    ASHIZAWA, K
    WISHART, GJ
    TOMONAGA, H
    NISHINAKAMA, K
    TSUZUKI, Y
    [J]. FEBS LETTERS, 1994, 350 (01) : 130 - 134
  • [5] DEPHOSPHORYLATION OF A 30-KDA PROTEIN OF FOWL SPERMATOZOA BY THE ADDITION OF MYOSIN LIGHT-CHAIN KINASE SUBSTRATE PEPTIDE INHIBITS THE FLAGELLAR MOTILITY
    ASHIZAWA, K
    WISHART, GJ
    HASHIMOTO, K
    TSUZUKI, Y
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 215 (02) : 706 - 712
  • [6] ASHIZAWA K, 1987, J REPROD FERTIL, V81, P495, DOI 10.1530/jrf.0.0810495
  • [7] ASHIZAWA K, 1989, J REPROD FERTIL, V86, P263, DOI 10.1530/jrf.0.0860263
  • [8] ASHIZAWA K, 1995, J REPROD FERTIL, V104, P141, DOI 10.1530/jrf.0.1040141
  • [9] Ashizawa K, 1978, JAP POULT SCI, V15, P264