A sequence-ready physical map of a region of 12q24.1

被引：9

作者：

Renault, B

Hovnanian, A

Bryce, S

Chang, JJ

Lau, S

Sakuntabhai, A

Monk, S

Carter, S

Ross, CJD

Pang, J

Twells, R

Chamberlain, S

Monaco, AP

Strachan, T

Kucherlapati, R

机构：

[1] WELLCOME TRUST CTR HUMAN GENET,OXFORD OX3 7BN,ENGLAND

[2] UNIV NEWCASTLE UPON TYNE,DEPT HUMAN GENET,NEWCASTLE TYNE NE1 7RU,TYNE & WEAR,ENGLAND

[3] ST MARYS HOSP,DEPT BIOCHEM & MOL GENET,SCH MED,LONDON W1PG,ENGLAND

来源：

GENOMICS | 1997年 / 45卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1006/geno.1997.4888

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We developed a sequence-ready map of a part of human chromosome 12q24.1. We utilized a number of sequence-tagged site (STS) markers hom 12q24.1 to screen large insert bacterial chromosome libraries and a chromosome la-specific cosmid library. The clones were assembled into contiguous sets (contigs) by STS-content analysis. Contigs were extended by obtaining end sequences of bacterial clones, generation of additional STSs, rescreening the libraries, and screening the additional clones for the presence of STSs. The resulting contig covers nearly 2 Mb of DNA and provides an average marker resolution of 16 kb. Based on the STS content, we developed fingerprints of a subset of clones. The STS content and fingerprint data allowed us to define a minimal thing path of clones. These clones are being used to sequence this part of chromosome 12. This contig contains the Ataxin 2 gene, and it covers the interval harboring the gene responsible for Darier disease. (C) 1997 Academic Press.

引用

页码：271 / 278

页数：8

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