Human septin-septin interaction:: CDCre1-1 partners with KIAA0202

被引：29

作者：

Bläser, S ^{[1
]}

Jersch, K ^{[1
]}

Hainmann, I ^{[1
]}

Wunderle, D ^{[1
]}

Zgaga-Griesz, A ^{[1
]}

Busse, A ^{[1
]}

Zieger, B ^{[1
]}

机构：

[1] Univ Freiburg, Childrens Hosp, D-79106 Freiburg, Germany

来源：

FEBS LETTERS | 2002年 / 519卷 / 1-3期

关键词：

septin; yeast two-hybrid assays; protein interaction; KIAA0202;

D O I：

10.1016/S0014-5793(02)02749-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Septins are evolutionary conserved cytoskeletal GTPases forming heteropolymer complexes involved in cytokinesis and other cellular processes. CDCrel-1 (cell division cycle related-1) is a recently cloned and characterized human septin which is highly expressed in non-dividing cells, such as neurons. Using a yeast two-hybrid system we demonstrate that CDCrel-1 partners with another uncharacterized human septin, KIAA0202. The interaction of CDCrel-1 and KIAA0202 was confirmed in the human leukemia cell line K-562 using pull-down assays with a KIAA0202-glutathione S-transferase fusion protein and by immunoprecipitation of the CDCrel-1-KIAA0202 complex with an anti-KIAA0202 antibody. Expression studies of the two human septins revealed a concomitant expression of both proteins in certain cells. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：169 / 172

页数：4

共 23 条

[1] ADAMS AEM, 1984, THESIS U MICHIGAN
[2] Bartel PL., 1993, Cellular interactions in development: A practical approach
[3] The septin CDCrel-1 binds syntaxin and inhibits exocytosis
Beites, CL
Xie, H
Bowser, R
Trimble, WS
[J]. NATURE NEUROSCIENCE, 1999, 2 (05) : 434 - 439
[4] Septin scaffolds and cleavage planes in Saccharomyces
Chant, J
[J]. CELL, 1996, 84 (02) : 187 - 190
[5] Septins: cytoskeletal polymers or signalling GTPases?
Field, CM
Kellogg, D
[J]. TRENDS IN CELL BIOLOGY, 1999, 9 (10) : 387 - 394
[6] A purified Drosophila septin complex forms filaments and exhibits GTPase activity
Field, CM
AlAwar, O
Rosenblatt, J
Wong, ML
Alberts, B
Mitchison, TJ
[J]. JOURNAL OF CELL BIOLOGY, 1996, 133 (03) : 605 - 616
[7] Polymerization of purified yeast septins: Evidence that organized filament arrays may not be required for septin function
Frazier, JA
Wong, ML
Longtine, MS
Pringle, JR
Mann, M
Mitchison, TJ
Field, C
[J]. JOURNAL OF CELL BIOLOGY, 1998, 143 (03) : 737 - 749
[8] GENETIC CONTROL OF CELL DIVISION CYCLE IN YEAST .4. GENES CONTROLLING BUD EMERGENCE AND CYTOKINESIS
HARTWELL, LH
[J]. EXPERIMENTAL CELL RESEARCH, 1971, 69 (02) : 265 - &
[9] Subunit composition, protein interactions, and structures of the mammalian brain sec6/8 complex and septin filaments
Hsu, SC
Hazuka, CD
Roth, R
Foletti, DL
Heuser, J
Scheller, RH
[J]. NEURON, 1998, 20 (06) : 1111 - 1122
[10] FINDING NEW GENES IN THE NERVOUS-SYSTEM BY CDNA ANALYSIS
KATO, K
[J]. TRENDS IN NEUROSCIENCES, 1992, 15 (09) : 319 - 323

← 1 2 3 →