Mixed alkanethiol self-assembled monolayers as substrates for microarraying applications

被引:9
作者
Datwani, SS
Vijayendran, RA
Johnson, E
Biondi, SA
机构
[1] Caliper Life Sci Inc, Mountain View, CA 94043 USA
[2] Stanford Univ, Dept Engn Mech, Stanford, CA 94305 USA
[3] Biosite Inc, San Diego, CA 92121 USA
[4] Eksigent Technol LLC, Livermore, CA 94551 USA
关键词
D O I
10.1021/la030140x
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In current microarraying experiments, data quality is in large part determined by the quality of the spots that compose the microarray. Since many microarrays are made with contact printing techniques, microarray spot quality is fundamentally linked to the surface characteristics of the microarray substrate. In this work, surface coatings, consisting of self-assembled monolayers (SAMs) of mixed alkanethiol molecules, were used to control the surface properties of the microarray substrate. X-ray photoelectron spectroscopy and equilibrium contact angle measurements were performed in order to confirm the chemical content and wettability of these surface coatings. To test their performance in microarraying applications, sample microarrays were printed on these mixed alkanethiol films and then characterized with a noncontact visual metrology system and a fluorescence scanner. This work demonstrates that utilizing mixed alkanethiol SAMs as a surface coating provides spatially homogeneous surface characteristics that are reproducible across multiple microarray substrates as well as within a substrate. In addition, this paper demonstrates that these films are stable and robust as they can maintain their surface characteristics over time. Overall, it is demonstrated that SAMs of mixed alkanethiols serve as a useful surface coating, which enhances spot and therefore data quality in microarraying applications.
引用
收藏
页码:4970 / 4976
页数:7
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