Cutting edge: Identification of GL50, a novel B7-like protein that functionally binds to ICOS receptor

被引：203

作者：

Ling, V ^{[1
]}

Wu, PW ^{[1
]}

Finnerty, HF ^{[1
]}

Bean, KM ^{[1
]}

Spaulding, V ^{[1
]}

Fouser, LA ^{[1
]}

Leonard, JP ^{[1
]}

Hunter, SE ^{[1
]}

Zollner, R ^{[1
]}

Thomas, JL ^{[1
]}

Miyashiro, JS ^{[1
]}

Jacobs, KA ^{[1
]}

Collins, M ^{[1
]}

机构：

[1] Genet Inst, Dept Immunol, Cambridge, MA 02081 USA

来源：

JOURNAL OF IMMUNOLOGY | 2000年 / 164卷 / 04期

关键词：

D O I：

10.4049/jimmunol.164.4.1653

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

By the genetic selection of mouse cDNAs encoding secreted proteins, a B7-like cDNA clone termed mouse GL50 (mGL50) was isolated encoding a 322-aa polypeptide identical with B7h. Isolation of the human ortholog of this cDNA (hGL50) revealed a coding sequence of 309 aa residues with 42% sequence identity with mGL50, Northern analysis indicated GL50 to be present in many tissues including lymphoid, embryonic yolk sac, and fetal liver samples. Of the CD28, CTLA4 and ICOS fusion constructs tested, flow cytometric analysis demonstrated only mouse ICOS-IgG binding to mGL50 cell transfectants. Subsequent phenotyping demonstrated high levels of ICOS ligand staining on splenic CD19(+) B cells and low levels on CD3(+) T cells. These results indicate that GL50 is a specific ligand for the ICOS receptor and suggest that the GL50-ICOS interaction functions in lymphocyte costimulation.

引用

页码：1653 / 1657

页数：5

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