Monoclonal antibodies against Streptococcus pneumoniae detect epitopes on eubacterial ribosomal proteins L7/L12 and on streptococcal elongation factor Ts

被引:32
作者
Kolberg, J
Hoiby, EA
Lopez, R
Sletten, K
机构
[1] NATL INST PUBL HLTH,DEPT BACTERIOL,N-0403 OSLO,NORWAY
[2] UNIV OSLO,CTR BIOTECHNOL,N-0371 OSLO,NORWAY
[3] UNIV OSLO,DEPT BIOCHEM,N-0371 OSLO,NORWAY
来源
MICROBIOLOGY-UK | 1997年 / 143卷
关键词
monoclonal antibodies; eubacteria; Streptococcus pneumoniae; ribosomal protein L7/L12; elongation factor Ts;
D O I
10.1099/00221287-143-1-55
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two monoclonal antibodies (mAbs) designated 144,H-3 (IgG2a) and 218,C-5 (IgM) were produced after immunization of mice with two different heat-treated and sonicated pneumococcal strains. Western blotting, with solubilized proteins from different bacterial genera and from mammalian lymphocytes, showed that both mAbs reacted with a protein of approximately 12 kDa in all 66 strains of eubacteria examined, representing 27 different species. The 12 kDa protein was isolated by immunoaffinity chromatography. Subsequent of preparative Western blotting enabled N-terminal amino acid sequence analysis by microsequencing. A high degree of amino acid sequence similarity with eubacterial ribosomal proteins L7/L12 was demonstrated. One of the mAbs (144,H-3) also cross-reacted in Western blotting with a 43 kDa protein, but only from streptococci. The 43 kDa protein carrying the common streptococcal epitope was isolated and sequenced in the N-terminal region. A high degree of amino acid sequence identity was found to elongation factor Ts from Escherichia coli.
引用
收藏
页码:55 / 61
页数:7
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