One-step enzyme-linked immunosorbent assay (ELISA) for plasma fatty acid-binding protein

被引：93

作者：

Wodzig, KWH

Pelsers, MMAL

vanderVusse, GJ

Roos, W

Glatz, JFC

机构：

[1] UNIV LIMBURG,CARDIOVASC RES INST MAASTRICHT,DEPT PHYSIOL,NL-6200 MD MAASTRICHT,NETHERLANDS

[2] ACAD HOSP MAASTRICHT,DEPT CLIN CHEM,MAASTRICHT,NETHERLANDS

[3] ROCHE DIAGNOST SYST,BASEL,SWITZERLAND

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1997年 / 34卷

关键词：

heart-type fatty acid-binding protein; monoclonal antibodies;

D O I：

10.1177/000456329703400307

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

To allow a more rapid determination of heart-type fatty acid-binding protein (FABP) concentration in plasma a direct non-competitive (sandwich-type) ELISA was developed which uses high-affinity monoclonal antibodies to FABP. Total performance time of the one-step immunoassay is 45 min. The standard curve was linear between 0.2-6 mu g/L, and the within-run and between-run coefficients of variations were below 6 and 11%, respectively. The serum FABP concentration measured in 79 healthy individuals was 1.6 (0.8) [mean (SD), range 0.3-5.0] mu g/L. The assay can be used for rapid plasma or serum FABP measurement in the early diagnosis of acute myocardial infarction.

引用

页码：263 / 268

页数：6

共 18 条

[1] BIOCHEMICAL MARKERS OF MYOCARDIAL INJURY - IS MB CREATINE-KINASE THE CHOICE FOR THE 1990S [J].