Rayleigh light-scattering spectroscopy: Application to the determination of proteins using bromopyrogallol red

被引：50

作者：

Ma, CQ ^{[1
]}

Li, KA ^{[1
]}

Tong, SY ^{[1
]}

机构：

[1] BEIJING UNIV, DEPT CHEM, BEIJING 100871, PEOPLES R CHINA

来源：

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN | 1997年 / 70卷 / 01期

关键词：

D O I：

10.1246/bcsj.70.129

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A method for the quantitation of proteins in aqueous solution involving the binding of bromopyrogallol red to proteins under acidic conditions has been developed. The binding of the dye to proteins is accompanied by an enhancement of Rayleigh light scattering at 332 nm; the scattering intensity is linear over the range 0.136-6.80 mu g ml(-1). The reaction is completed immediately after mixing dye and protein solutions, and the scattering signal is stable for at least 3 h. There are very few interference with the method, most of which can be minimized by dilution.

引用

页码：129 / 133

页数：5

共 20 条

[1] DEPOLARIZED RAYLEIGH LIGHT-SCATTERING IN ABSORPTION-BANDS MEASURED IN LYCOPENE SOLUTION [J].

ANGLISTER, J ;

STEINBERG, IZ .

CHEMICAL PHYSICS LETTERS, 1979, 65 (01) :50-54

[2] MEASUREMENT OF THE RESONANTLY ENHANCED QUASIELASTIC LIGHT-SCATTERING CROSS-SECTIONS OF HEME-PROTEINS [J].

CHIARELLO, R ;

REINISCH, L .

JOURNAL OF CHEMICAL PHYSICS, 1988, 88 (02) :1253-1259

[3] AGGREGATION OF CHLOROPHYLL-A PROBED BY RESONANCE LIGHT-SCATTERING SPECTROSCOPY [J].

DEPAULA, JC ;

ROBBLEE, JH ;

PASTERNACK, RF .

BIOPHYSICAL JOURNAL, 1995, 68 (01) :335-341

[4]

FASMAN GD, 1976, CRC PRACTICAL HDB BI, V2

[5] THE DETERMINATION OF A SMALL AMOUNT OF BIOLOGICAL CONSTITUENT BY THE USE OF CHEMI-LUMINESCENCE .13. HIGH SENSITIVE METAL CHELATE AFFINITY-CHROMATOGRAPHY [J].

HARA, T ;

TSUKAGOSHI, K ;

YOSHIDA, T .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1988, 61 (08) :2779-2783

[6] THE DETERMINATION OF A SMALL AMOUNT OF BIOLOGICAL CONSTITUENT BY THE USE OF CHEMI-LUMINESCENCE .14. THE FLOW-INJECTION ANALYSIS OF PROTEIN USING ULTRASONIC CHEMI-LUMINESCENCE OF LUMINOL [J].

HARA, T ;

NAKATSU, K ;

ARAI, A ;

YOSHIDA, T ;

TSUKAGOSHI, K .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1988, 61 (08) :2996-2998

[7] FLUOROMETRIC ASSAY OF PROTEIN IN NATIVE HUMAN BILE [J].

HARVEY, PRC ;

UPADHYA, GA ;

TOTH, JL ;

STRASBERG, SM .

CLINICA CHIMICA ACTA, 1989, 183 (02) :147-154

[8]

HELENA SS, 1994, ANAL BIOCHEM, V220, P142

[9] Determination of nucleic acids by a resonance light-scattering technique with alpha,beta,gamma,delta-tetrakis[4-(trimothylammoniumyl)phenyl]porphine [J].

Huang, CZ ;

Li, KA ;

Tong, SY .

ANALYTICAL CHEMISTRY, 1996, 68 (13) :2259-2263

[10]

KIRSCHENBAUM DM, 1990, HDB BIOCH SELECTED D, pC71

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