Artemisinin production in Artemisia annua hairy root cultures with improved growth by altering the nitrogen source in the medium

被引：42

作者：

Wang, JW ^{[1
]}

Tan, RX ^{[1
]}

机构：

[1] Nanjing Univ, Inst Funct Biomol, Nanjing 210093, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 14期

关键词：

ammonium; Artemisia annua; artemisinin; hairy roots; nitrate;

D O I：

10.1023/A:1016126917795

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Murashige & Skoog medium was modified for enhancing artemisinin production in Artemisia annua hairy root cultures by altering the ratio of NO3-/NH4+ and the total amount of initial nitrogen. Increasing ammonium to 60 mM decreased both growth and artemisinin accumulation in hairy root cultures. With NO3-/NH4+ at 5: 1 (w/w), the optimum concentration of total initial nitrogen for artemisinin production was 20 mM. After 24 days of cultivation with 16.7 mM nitrate and 3.3 mM ammonium, the maximum artemisinin production of hairy roots was about 14 mg l(-1), a 57% increase over that in the standard MS medium.

引用

页码：1153 / 1156

页数：4

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