Evaluation of a commercial enzyme immunoassay for the detection of norovirus antigen in fecal samples from children with sporadic acute gastroenteritis

被引：9

作者：

Gonzalez, G. G.

Liprandi, F.

Ludert, J. E.

机构：

[1] Inst Venezolano Invest Cient, Lab Biol Virus, Ctr Microbiol & Biol Celular, Caracas 1020A, Venezuela

[2] Univ Carabobo, Escuela Bioanal, Dept Microbiol, Valencia, Spain

来源：

JOURNAL OF VIROLOGICAL METHODS | 2006年 / 136卷 / 1-2期

关键词：

norovirus; EIA; (RT)-PCR; diarrhea;

D O I：

10.1016/j.jviromet.2006.05.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Ridascreen (R) Norwalk-like virus enzyme immunoassay was compared with (RT)-PCR on 92 stool samples collected from children with sporadic acute gastroenteritis. Homogenization and pre-dilution of the whole stool sample resulted in high specificity (97.5%) and moderate sensitivity (60%). This assay may be useful to screen outbreaks for norovirus, but limited to detect the virus in sporadic cases of diarrhea. (c) 2006 Elsevier B.V. All rights reserved.

引用

收藏

页码：289 / 291

页数：3

相关论文

共 17 条

[1] Identification of viral agents causing gastroenteritis among children in Buenos Aires, Argentina [J].

Bereciartu, A ;

Bok, K ;

Gómez, J .

JOURNAL OF CLINICAL VIROLOGY, 2002, 25 (02) :197-203

[2] Etiology of sporadic cases of pediatric acute gastroenteritis in Asturias, Spain, and genotyping and characterization of norovirus strains involved [J].

Boga, JA ;

Melón, S ;

Nicieza, I ;

de Diego, I ;

Villar, M ;

Parra, F ;

de Oña, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (06) :2668-2674

[3] Evaluation and comparison of two commercial enzyme-linked immunosorbent assay kits for detection of antigenically diverse human noroviruses in stool samples [J].

Burton-MacLeod, JA ;

Kane, EM ;

Beard, RS ;

Hadley, LA ;

Glass, RI ;

Ando, T .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (06) :2587-2595

[4] A review of viral gastroenteritis [J].

Clark, B ;

McKendrick, M .

CURRENT OPINION IN INFECTIOUS DISEASES, 2004, 17 (05) :461-469

[5] Evaluation of a commercial enzyme immunoassay for detection of norovirus in outbreak specimens [J].

Dimitriadis, A ;

Marshall, JA .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2005, 24 (09) :615-618

[6] POLYMERASE CHAIN-REACTION DETECTION OF SMALL ROUND-STRUCTURED VIRUSES FROM 2 RELATED HOSPITAL OUTBREAKS OF GASTROENTERITIS USING INOSINE-CONTAINING PRIMERS [J].

GREEN, SM ;

LAMBDEN, PR ;

DENG, Y ;

LOWES, JA ;

LINEHAM, S ;

BUSHELL, J ;

ROGERS, J ;

CAUL, EO ;

ASHLEY, CR ;

CLARKE, IN .

JOURNAL OF MEDICAL VIROLOGY, 1995, 45 (02) :197-202

[7]

Jiang X, 2003, PERSP MED V, V9, P505

[8] MEASUREMENT OF OBSERVER AGREEMENT FOR CATEGORICAL DATA [J].

LANDIS, JR ;

KOCH, GG .

BIOMETRICS, 1977, 33 (01) :159-174

[9]

NEGISHI SO, 2004, PEDIATR INT, V46, P245

[10]

Pujol FH, 1998, ANN TROP MED PARASIT, V92, P205, DOI 10.1080/00034989860058