Fluorescence microscopy of single autofluorescent proteins for cellular biology

被引：13

作者：

Cognet, L

Coussen, F

Choquet, D

Lounis, B

机构：

[1] CNRS, UMR 5798, Ctr Phys Mol Opt & Hertzienne, F-33405 Talence, France

[2] Univ Bordeaux 1, F-33405 Talence, France

[3] CNRS, UMR 5091, Lab Physiol Cellulaire Synapse, F-33077 Bordeaux, France

[4] Univ Bordeaux 2, Inst Francois Magendie, F-33077 Bordeaux, France

来源：

COMPTES RENDUS PHYSIQUE | 2002年 / 3卷 / 05期

关键词：

single-molecule; GFP; DsRed; fluorescence microscopy; two-photon excitation; fluorescence correlation spectroscopy;

D O I：

10.1016/S1631-0705(02)01341-5

中图分类号：

P1 [天文学];

学科分类号：

0704 ;

摘要：

In this paper we review the applicability of autofluorescent proteins for single-molecule imaging in biology. The photophysical characteristics of several mutants of the Green Fluorescent Protein (GFP) and those of DsRed are compared and critically discussed for their use in cellular biology. The alternative use of two-photon excitation at the single-molecule level or Fluorescence Correlation Spectroscopy is envisaged for the study of individual autofluorescent proteins. Single-molecule experiments performed in live cells using eGFP and preferably eYFP fusion proteins are reviewed. Finally, the first use at the single-molecule level of citrine, a more photostable variant of the eYFP is reported when fused to a receptor for neurotransmitter in live cells. (C) 2002 Academic des sciences/Editions scientifiques et medicales Elsevier SAS.

引用

页码：645 / 656

页数：12

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