Genetic address book for retinal cell types

被引:150
作者
Siegert, Sandra [1 ]
Scherf, Brigitte Gross [1 ]
Del Punta, Karina [2 ]
Didkovsky, Nick [2 ]
Heintz, Nathaniel [2 ,3 ]
Roska, Botond [1 ]
机构
[1] Friedrich Miescher Inst Biomed Res, Neural Circuit Labs, Basel, Switzerland
[2] Rockefeller Univ, GENSAT Project, New York, NY 10021 USA
[3] Rockefeller Univ, Howard Hughes Med Inst, Mol Biol Lab, New York, NY 10021 USA
关键词
STARBURST AMACRINE CELLS; GANGLION-CELLS; MOUSE RETINA; BIPOLAR CELLS; DIRECTIONAL SELECTIVITY; MAMMALIAN RETINA; CAT RETINA; IN-VIVO; EXPRESSION; LIGHT;
D O I
10.1038/nn.2370
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The mammalian brain is assembled from thousands of neuronal cell types that are organized in distinct circuits to perform behaviorally relevant computations. Transgenic mouse lines with selectively marked cell types would facilitate our ability to dissect functional components of complex circuits. We carried out a screen for cell type-specific green fluorescent protein expression in the retina using BAC transgenic mice from the GENSAT project. Among others, we identified mouse lines in which the inhibitory cell types of the night vision and directional selective circuit were selectively labeled. We quantified the stratification patterns to predict potential synaptic connectivity between marked cells of different lines and found that some of the lines enabled targeted recordings and imaging of cell types from developing or mature retinal circuits. Our results suggest the potential use of a stratification-based screening approach for characterizing neuronal circuitry in other layered brain structures, such as the neocortex.
引用
收藏
页码:1197 / U30
页数:10
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