Detection of the Helicobacter pylori dupA gene is strongly affected by the PCR design

被引：4

作者：

Abadi, Amin Talebi Bezmin ^{[1
]}

Loffeld, Ruud J. L. F. ^{[2
]}

Constancia, Ashandra C. ^{[1
]}

Wagenaar, Jaap A. ^{[3
]}

Kusters, Johannes G. ^{[1
]}

机构：

[1] Univ Med Ctr Utrecht, Dept Med Microbiol, NL-3584 CX Utrecht, Netherlands

[2] Zaans Med Ctr, Zaandam Dept Internal Med, Zaandam, Netherlands

[3] Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Utrecht, Netherlands

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2014年 / 106卷

关键词：

Helicobacter pylori; dupA; Primer sets; ULCER-PROMOTING GENE; GASTRODUODENAL DISEASES; DUODENAL-ULCER; ASSOCIATION; POPULATION; PREVALENCE; INFECTION; STRAINS;

D O I：

10.1016/j.mimet.2014.07.027

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

The Helicobacter pylori virulence gene dupA is usually detected by PCR, but the primer binding sites used are highly variable. Our newly designed qPCR against a conserved region of dupA was positive in 64.2% of 394 clinical isolates while the positivity rate of the commonly used PCRs ranged from 29.9% to 37.8%. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：55 / 56

页数：2

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