Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli

被引：182

作者：

Millard, CS

Chao, YP

Liao, JC

Donnelly, MI

机构：

[1] ARGONNE NATL LAB,DIV ENVIRONM RES,ARGONNE,IL 60439

[2] TEXAS A&M UNIV,DEPT CHEM ENGN,COLLEGE STN,TX 77843

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 05期

关键词：

D O I：

10.1128/AEM.62.5.1808-1810.1996

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fermentative production of succinic acid from glucose by Escherichia coli was significantly increased by overexpression of phosphoenolpyruvate carboxylase. In contrast, overexpression of phosphoenolpyruvate carboxykinase had no effect. Under optimized conditions, induction of the carboxylase resulted in a 3.5-fold increase in the concentration of succinic acid, mailing succinic acid the major fermentation product by weight.

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页码：1808 / 1810

页数：3

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