A 4.5-megabase YAC contig and physical map over the hemochromatosis gene region

被引：32

作者：

Burt, MJ ^{[1
]}

Smit, DJ ^{[1
]}

Pyper, WR ^{[1
]}

Powell, LW ^{[1
]}

Jazwinska, EC ^{[1
]}

机构：

[1] UNIV QUEENSLAND,QUEENSLAND INST MED RES,LIVER UNIT,BRISBANE,QLD 4029,AUSTRALIA

来源：

GENOMICS | 1996年 / 33卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1006/geno.1996.0178

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have constructed a yeast artificial chromosome (YAC) contig over the candidate hemochromatosis gene region. This contig comprises 16 YACs from the CEPH, Washington University, and ICI YAC libraries and covers 4.5 Mb at 6p21.3-6p22. The complete contig has been restriction mapped, enabling the precise relationship between the YACs to be determined and the mapping of a total of 12 STSs. Nine of these are highly polymorphic STSs that are closely linked to hemochromatosis; this series includes D6S265 and D6S1260, which comprise the most proximal and distal markers linked to HC. This is the first YAC contig that spans the hemochromatosis candidate region, and it provides valuable resource material for the cloning of this and other genes in the region distal to the MHC class I complex. (C) 1996 Academic Press, Inc.

引用

页码：153 / 158

页数：6

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