Identification and isolation of a pectin methylesterase isoform that could be involved in flax cell wall stiffening

被引：55

作者：

Al-Qsous, S ^{[1
]}

Carpentier, E ^{[1
]}

Klein-Eude, D ^{[1
]}

Burel, C ^{[1
]}

Mareck, A ^{[1
]}

Dauchel, H ^{[1
]}

Gomord, V ^{[1
]}

Balangé, AP ^{[1
]}

机构：

[1] Univ Rouen, Lab Signaux & Regulat Chez Vegetaux, CNRS, UMR 6307,IFRMP 23, F-76821 Mont St Aignan, France

来源：

PLANTA | 2004年 / 219卷 / 02期

关键词：

cell wall; isoform; Linum; pectin methylesterase; wall stiffening;

D O I：

10.1007/s00425-004-1246-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Pectin methylesterases (PMEs) are ubiquitous enzymes present in the plant cell wall. They catalyse the demethylesterification of homogalacturonic acid units of pectins, which, in turn, can be associated with different physiological phenomena. In this study, different flax (Linum usitatissimum L.) PME isoforms were observed: neutral (pI 7.0 and 7.5, MW: 110 kDa), basic (pI 8.3 and 8.5, MW: 110 kDa) and very basic (pI>9.5, MW: 38 kDa). In an attempt to identify most of the expressed cell wall LuPME isoforms, polyclonal antibodies were raised against a conserved region of PME. These antibodies allowed the purification of the very basic PME isoform (pI 9.5, MW: 36 kDa) from flax cells, designated LuPME5. This isoform corresponds to the Lupme5 cDNA isolated, at the same time, from flax hypocotyls, by using the RACE-PCR technique. Semi-quantitative PCR experiments showed that the Lupme5 transcript was highly expressed in the hypocotyl zones where elongation is being achieved. Thus, this enzyme may be involved in cell wall stiffening.

引用

页码：369 / 378

页数：10

共 44 条

[1] A GENE SHOWING SEQUENCE SIMILARITY TO PECTIN ESTERASE IS SPECIFICALLY EXPRESSED IN DEVELOPING POLLEN OF BRASSICA-NAPUS - SEQUENCES IN ITS 5' FLANKING REGION ARE CONSERVED IN OTHER POLLEN-SPECIFIC PROMOTERS [J].